User:Jarle Pahr/Kits: Difference between revisions
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! Kit!! Use !! Advantages !! Disadvantages !! Time !! Experience | ! Kit!! Use !! Advantages !! Disadvantages !! Time !! Experience | ||
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| Promega Wizard SV miniprep || DNA isolation || Quick || Low yield || || | | Promega Wizard SV miniprep || DNA isolation || Quick || Low yield || || | ||
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| QIAEX II gel extraction|| DNA extraction from gel || Higher purity than qiaquick || Slower than Quiaquick|| || | | QIAEX II gel extraction|| DNA extraction from gel || Higher purity than qiaquick || Slower than Quiaquick|| || | ||
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|[http://www.qiagen.com/Products/Catalog/Assay-Technologies/End-Point-PCR-and-RT-PCR-Reagents/QIAGEN-PCR-Cloning-plus-Kit#productdetails QIAGEN PCR Cloningplus Kit]||Subcloning (TA cloning)||?||?|| ?|| | |||
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Revision as of 07:51, 11 March 2013
Commercial kits for applications in molecular biology:
HisExpress protein purification columns: http://www.claremontbio.com/HisExpress_Protein_Purification_Columns_s/72.htm
(available at http://www.bioquote.com/product.php?id=291680)
PrepEase his-purification: http://www.affymetrix.com/estore/browse/brand/usb/product.jsp?navMode=34000&productId=131366&navAction=jump&aId=productsNav#1_1
Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg
| Kit | Use | Advantages | Disadvantages | Time | Experience |
|---|---|---|---|---|---|
| Promega Wizard SV miniprep | DNA isolation | Quick | Low yield | ||
| Machery-Nagel NucleoBond Xtra Midi (midiprep) | DNA isolation | High yield (~20 ug) | Slow | 2 d | |
| Qiaquick gel extraction | DNA extraction from gel | Fast | Impure product | ||
| Qiaquick PCR purification | Purification of PCR products | Fast | |||
| QIAEX II gel extraction | DNA extraction from gel | Higher purity than qiaquick | Slower than Quiaquick | ||
| QIAGEN PCR Cloningplus Kit | Subcloning (TA cloning) | ? | ? | ? |
