User:Jarle Pahr/Kits: Difference between revisions
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|[http://no.promega.com/resources/protocols/technical-bulletins/101/pureyield-plasmid-miniprep-system-protocol/ Promega PureYield™ Plasmid Miniprep System]||High-purity DNA isolation|| || || || | |[http://no.promega.com/resources/protocols/technical-bulletins/101/pureyield-plasmid-miniprep-system-protocol/ Promega PureYield™ Plasmid Miniprep System]||High-purity DNA isolation|| || || || | ||
|- | |- | ||
| Machery-Nagel NucleoBond Xtra Midi (midiprep)|| DNA isolation ||High yield (~20 ug), pure product. || Slow || 2 d || Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet. | | Machery-Nagel NucleoBond Xtra Midi (midiprep)|| DNA isolation ||High yield (~20 ug), pure product. || Slow || 2 d || Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet. Might be more efficient to do several (~10) parallel minipreps instead? | ||
|- | |- | ||
| Qiaquick gel extraction|| DNA extraction from gel || Fast|| Impure product || ||Impurities give absorbption peak at 230 nm in eluted product. | | Qiaquick gel extraction|| DNA extraction from gel || Fast|| Impure product || ||Impurities give absorbption peak at 230 nm in eluted product. | ||
Revision as of 14:00, 24 March 2013
Commercial kits for applications in molecular biology:
HisExpress protein purification columns: http://www.claremontbio.com/HisExpress_Protein_Purification_Columns_s/72.htm
(available at http://www.bioquote.com/product.php?id=291680)
PrepEase his-purification: http://www.affymetrix.com/estore/browse/brand/usb/product.jsp?navMode=34000&productId=131366&navAction=jump&aId=productsNav#1_1
Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg
| Kit | Use | Advantages | Disadvantages | Time | Experience |
|---|---|---|---|---|---|
| Promega Wizard SV miniprep | DNA isolation | Quick | Low yield | ||
| Promega PureYield™ Plasmid Miniprep System | High-purity DNA isolation | ||||
| Machery-Nagel NucleoBond Xtra Midi (midiprep) | DNA isolation | High yield (~20 ug), pure product. | Slow | 2 d | Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet. Might be more efficient to do several (~10) parallel minipreps instead? |
| Qiaquick gel extraction | DNA extraction from gel | Fast | Impure product | Impurities give absorbption peak at 230 nm in eluted product. | |
| Qiaquick PCR purification | Purification of PCR products | Fast | |||
| QIAEX II gel extraction | DNA extraction from gel | Higher purity than qiaquick | Slower than Quiaquick | Yield of about 200 ng from ~1.5 ug starting amount of plasmid. | |
| QIAGEN PCR Cloningplus Kit | Subcloning (TA cloning) | ? | ? | ? | |
| Dongsheng Bio Eco-friendly Plasmid miniprep kit | DNA isolation | ? | - | ||
| Dongsheng Bio PCR and DNA Fragment Purification Kit | Purification of PCR fragments | ? | |||
| QiaAmp |
