User:Jarle Pahr/Growth media

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Luria Broth (LB):
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Luria Broth/Lysogeny Broth (LB):
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http://en.wikipedia.org/wiki/Lysogeny_broth

Revision as of 10:01, 21 May 2013

Compositions of various growth media:


Luria Broth/Lysogeny Broth (LB):

http://en.wikipedia.org/wiki/Lysogeny_broth


In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.) According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3


LA:

To make LA plates, add 20 g agar/L

Psi-B medium:


M9 minimal medium:


Bibliograpy:


Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full

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