User:Jarle Pahr/Growth media

Compositions of various growth media:

Luria Broth/Lysogeny Broth (LB):

http://en.wikipedia.org/wiki/Lysogeny_broth

In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.) According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3

LA:

To make LA plates, add 20 g agar/L

Psi-B medium:

M9 minimal medium:

Bibliograpy:

http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html

Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full