User:Jarle Pahr/Growth media: Difference between revisions
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Luria Broth (LB): | Luria Broth/Lysogeny Broth (LB): | ||
http://en.wikipedia.org/wiki/Lysogeny_broth | |||
Revision as of 07:01, 21 May 2013
Compositions of various growth media:
Luria Broth/Lysogeny Broth (LB):
http://en.wikipedia.org/wiki/Lysogeny_broth
In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.)
According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3
LA:
To make LA plates, add 20 g agar/L
Psi-B medium:
M9 minimal medium:
Bibliograpy:
Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full
