User:Jarle Pahr/Growth media: Difference between revisions

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In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.)
In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.)
According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3





Revision as of 03:37, 21 May 2013

Compositions of various growth media:


Luria Broth (LB):



In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.) According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3


LA:

To make LA plates, add 20 g agar/L

Psi-B medium:


M9 minimal medium:


Bibliograpy:


Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full