Notes on GFP and related proteins:
- Contains figure of a GFP sensor setup.
A Bilirubin-Inducible Fluorescent Protein from Eel Muscle: http://www.sciencedirect.com/science/article/pii/S0092867413006442
|GFP (A. wt)|
Lu et al. 2004. A high-throughput approach to promoter study using green fluorescent protein. http://www.ncbi.nlm.nih.gov/pubmed/15575693
- GFP expression experiments in 96-well format.
Differential rates of gene expression monitored by green fluorescent protein: http://onlinelibrary.wiley.com/doi/10.1002/bit.10295/abstract
Trezzani et al. 2003. Monitoring of recombinant protein production using bioluminescence in a semiautomated fermentation process. http://www.ncbi.nlm.nih.gov/pubmed/12892506
Predictive and interpretive simulation of green fluorescent protein expression in reporter bacteria: http://jb.asm.org/content/183/23/6752
Chae et al. 2000. Framework for online optimization of recombinant protein expression in high-cell-density Escherichia coli cultures using GFP-fusion monitoring: http://www.ncbi.nlm.nih.gov/pubmed/10861407
Randers-Eichorn and Albano 1997. On-line green fluorescent protein sensor with LED excitation. http://www.ncbi.nlm.nih.gov/pubmed/18636601
2011. Fluorescent proteins at a glance. Journal of Cell Science. http://jcs.biologists.org/content/124/2/157.full
Chong 2010. Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses: Potential and Challenges. Chinese Journal of Chemical Engineering. Volume 18, Issue 5, October 2010, Pages 863–869 http://www.sciencedirect.com/science/article/pii/S1004954109601403#
2009. Engineered fluorescent proteins: innovations and applications. Nature Methods. http://www.nature.com/nmeth/journal/v6/n10/abs/nmeth1009-713.html
2007. Advances in fluorescent protein technology. Journal of Cell Science. http://jcs.biologists.org/content/120/24/4247.abstract
Fluorescent proteins: maturation, photochemistry and photophysics. Curr Opin Struct Biol. 2006 Dec;16(6):714-21. Epub 2006 Oct 24. http://www.sciencedirect.com/science/article/pii/S0959440X06001734
2003. Biotechnological applications of green fluorescent protein. http://www.ncbi.nlm.nih.gov/pubmed/12768245
Zimmer 2002. Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. http://www.ncbi.nlm.nih.gov/pubmed/11890756
Understanding, improving and using green fluorescent proteins. Trends Biochem Sci. 1995 Nov;20(11):448-55. http://www.ncbi.nlm.nih.gov/pubmed/8578587
Dual Labeling with Green Fluorescent Proteins for Confocal Microscopy. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91838/
2002. Chem Rev. Green Fluorescent Protein (GFP): Applications, Structure, and Related Photophysical Behavior. 102, 759−781. http://pubs.acs.org/doi/pdf/10.1021/cr010142r
2001.Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence. Anal Biochem. 2001 Apr 15;291(2):175-97. http://www.ncbi.nlm.nih.gov/pubmed/11401292.
1998. Albano et al. Green fluorescent protein as a real time quantitative reporter of heterologous protein production. http://www.ncbi.nlm.nih.gov/pubmed/9548792
1998. The Green Fluorescent Protein. Annu. Rev. Biochem. 1998. 67:509–44. http://www.annualreviews.org/doi/pdf/10.1146/annurev.biochem.67.1.509
1997. Chromophore formation in green fluorescent protein. Biochemistry. 1997 Jun 3;36(22):6786-91. http://www.ncbi.nlm.nih.gov/pubmed/9184161.
"One potential limitation of GFP as a reporter for gene expression is its relatively slow rate of fluorescence acquisition in vivo limiting its use to experiments exceeding the hour time scale. The slow rate of fluorescence acquisition is reported to be due to the oxidation step in chromophore formation (Figure 1), which has been estimated to occur with a time constant of 2-4 h in vivo. (Heim et al., 1994)"
1997. Applications of the green fluorescent protein in cell biology and biotechnology. Nature Biotechnology 15, 961 - 964 (1997) doi:10.1038/nbt1097-961. http://www.nature.com/nbt/journal/v15/n10/abs/nbt1097-961.html
1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Current Biology Volume 6, Issue 2, February 1996, Pages 178–182. http://www.sciencedirect.com/science/article/pii/S0960982202004505
Crystal Structure of the Aequorea victoria Green Fluorescent Protein. Science 6 September 1996: Vol. 273 no. 5280 pp. 1392-1395. http://www.sciencemag.org/content/273/5280/1392.short (BP;DR)
Albano et al. 1996. Quantitative measurement of green fluorescent protein expression http://link.springer.com/article/10.1007%2FBF00180401
1995. GREEN FLUORESCENT PROTEIN. Photochemistry and Photobiology Volume 62, Issue 4, pages 651–656, October 1995 http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.1995.tb08712.x/abstract
Heim, Cubitt, Tsien 1995. Improved green fluorescence. http://www.nature.com/nature/journal/v373/n6516/abs/373663b0.html
Heim et al. 1994. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. http://www.pnas.org/content/91/26/12501.short Science 11 February 1994: Vol. 263 no. 5148 pp. 802-805
1994. Green fluorescent protein as a marker for gene expression. http://www.sciencemag.org/content/263/5148/802.short
Inouye and Tsuji 1994. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein.. http://www.ncbi.nlm.nih.gov/pubmed/8137953
Using GFP to see the light. Technical focus, Trends in Genetics Volume 11, Issue 8, Pages 295-334 (August 1995) http://www.sciencedirect.com/science/article/pii/S0168952500890903
1992. Primary structure of the Aequorea victoria green-fluorescent protein. Gene, 111(2), 229-233 (1992). http://www.ncbi.nlm.nih.gov/pubmed/1347277