User:Jarle Pahr/Electrophoresis

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Current revision (14:15, 30 August 2014) (view source)
 
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Lysplate:
Lysplate:
http://www.kptkomet.no/produkter/no_89/biologi/vnr/790510/
http://www.kptkomet.no/produkter/no_89/biologi/vnr/790510/
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=Equipment=
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WVR:
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Thermo scientific:
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http://www.thermoscientific.com/content/tfs/en/product/owl-easycast-b1-mini-gel-electrophoresis-systems.html
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Carolina:

Current revision

Notes on gel electrophoresis

Plasmid DNA that has not been cut with a restriction endonuclease will migrate in 3 forms: supercoiled closed circular DNA, single-strand nicked open circle DNA, and sheared linear DNA. http://www.sfu.ca/biology/courses/bisc431/lab3.htm

Supercoiled plasmid DNA will migrate faster than linear forms.

The effect of loading dye may cause mismatch between sample DNA bands and the DNA ladder bands.


http://www.protocol-online.org/biology-forums/agarose.html


Supplies

Lysplate: http://www.kptkomet.no/produkter/no_89/biologi/vnr/790510/


Equipment

WVR:


Thermo scientific:

http://www.thermoscientific.com/content/tfs/en/product/owl-easycast-b1-mini-gel-electrophoresis-systems.html


Carolina:


Visualization

http://www.bluetransilluminator.com/

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