User:Jarle Pahr/Electrophoresis: Difference between revisions
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Plasmid DNA that has not been cut with a restriction endonuclease will migrate in 3 forms: supercoiled closed circular DNA, single-strand nicked open circle DNA, and sheared linear DNA. http://www.sfu.ca/biology/courses/bisc431/lab3.htm | Plasmid DNA that has not been cut with a restriction endonuclease will migrate in 3 forms: supercoiled closed circular DNA, single-strand nicked open circle DNA, and sheared linear DNA. http://www.sfu.ca/biology/courses/bisc431/lab3.htm | ||
Supercoiled plasmid DNA will migrate faster than linear forms. | |||
The effect of loading dye may cause mismatch between sample DNA bands and the DNA ladder bands. | |||
http://www.protocol-online.org/biology-forums/agarose.html | |||
=Supplies= | |||
Lysplate: | |||
http://www.kptkomet.no/produkter/no_89/biologi/vnr/790510/ | |||
=Equipment= | |||
WVR: | |||
Thermo scientific: | |||
http://www.thermoscientific.com/content/tfs/en/product/owl-easycast-b1-mini-gel-electrophoresis-systems.html | |||
Carolina: | |||
=Visualization= | |||
http://www.bluetransilluminator.com/ | |||
Latest revision as of 11:15, 30 August 2014
Notes on gel electrophoresis
Plasmid DNA that has not been cut with a restriction endonuclease will migrate in 3 forms: supercoiled closed circular DNA, single-strand nicked open circle DNA, and sheared linear DNA. http://www.sfu.ca/biology/courses/bisc431/lab3.htm
Supercoiled plasmid DNA will migrate faster than linear forms.
The effect of loading dye may cause mismatch between sample DNA bands and the DNA ladder bands.
http://www.protocol-online.org/biology-forums/agarose.html
Supplies
Lysplate: http://www.kptkomet.no/produkter/no_89/biologi/vnr/790510/
Equipment
WVR:
Thermo scientific:
Carolina:
