User:Jarle Pahr/DNA

Analysis http://nucleicacids.bitesizebio.com/articles/how-to-identify-supercoils-nicks-and-circles-in-plasmid-preps/

Quick reference - Determining DNA concentration and purity: http://nucleicacids.bitesizebio.com/articles/dna-concentration-purity/

Isolation:

DNA isolation methods anno 1999: http://rothlab.ucdavis.edu/protocols/DNA-from-Agarose-Review.html

Gel extraction by glass wool filtering: http://www.jcancer.org/v03p0093.htm

http://nucleicacids.bitesizebio.com/articles/10-tips-for-better-dna-gel-extraction-results/

http://nucleicacids.bitesizebio.com/articles/5-more-tips-for-dna-gel-extraction/

How alkalyine lysis works: http://bitesizebio.com/articles/the-basics-how-alkaline-lysis-works/

How to get better midiprep yields: http://nucleicacids.bitesizebio.com/articles/how-to-get-better-plasmid-midiprep-yields/

A menagerie of miniprep methods: http://bitesizebio.com/articles/a-menagerie-of-mini-prep-methods/

http://www.ncbi.nlm.nih.gov/pubmed/388356

A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457

http://nucleicacids.bitesizebio.com/articles/how-to-maximize-the-quantity-and-quality-of-purified-plasmids/

http://nucleicacids.bitesizebio.com/articles/better-plasmid-midipreps-part-ii-what-causes-low-yields/

http://nucleicacids.bitesizebio.com/articles/tech-clinic-3-dna-digestion-precipitation-and-clean-up/