User:Jarle Pahr/Checklists: Difference between revisions
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Transformation: | '''Transformation:''' | ||
*Enough plates, with right antibiotic, are available | *Enough plates, with right antibiotic, are available | ||
*Waterbath for heat shock is at right temperature (42 C). | *Waterbath for heat shock is at right temperature (42 C). | ||
| Line 9: | Line 9: | ||
Restriction digest: | '''Restriction digest:''' | ||
*Needed restriction enzymes are available. | *Needed restriction enzymes are available. | ||
*Thaw buffer | *Thaw buffer | ||
| Line 15: | Line 15: | ||
'''Ligation:''' | |||
*Ligation buffer is available. | |||
*Ligase is available. | |||
*Correct ligase is used (T4 DNA ligase) | |||
Preparing supercompetent cells: | |||
'''Preparing supercompetent cells:''' | |||
*Centrifuge is cooled | *Centrifuge is cooled | ||
*Spectrophotometer and cuvettes for measuring OD are available | *Spectrophotometer and cuvettes for measuring OD are available | ||
Revision as of 05:11, 4 May 2013
Checklists for lab procedures:
Transformation:
- Enough plates, with right antibiotic, are available
- Waterbath for heat shock is at right temperature (42 C).
Before heat shock: Place plates in incubator for pre-heating.
Restriction digest:
- Needed restriction enzymes are available.
- Thaw buffer
- Waterbath at 37 C is available. (or otherwise, thermomixer, or otherwise, shaking incubator)
Ligation:
- Ligation buffer is available.
- Ligase is available.
- Correct ligase is used (T4 DNA ligase)
Preparing supercompetent cells:
- Centrifuge is cooled
- Spectrophotometer and cuvettes for measuring OD are available
- When using spectrophotometer: Correct wavelength is selected (600 nm)
- For snap freezing: Dry ice or liquid nitrogen is available.
- Freezer box is available
