User:Jarle Pahr/Checklists: Difference between revisions
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Jarle Pahr (talk | contribs) (New page: Checklists for lab procedures: Transformation: *Enough plates, with right antibiotic, are available Restriction digest: *Thaw buffer Preparing supercompetent cells: *Centrifuge...) |
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Checklists for lab procedures: | Checklists for lab procedures: | ||
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Transformation: | Transformation: | ||
*Enough plates, with right antibiotic, are available | *Enough plates, with right antibiotic, are available | ||
*Waterbath for heat shock is at right temperature (42 C). | |||
Before heat shock: Place plates in incubator for pre-heating. | |||
Restriction digest: | Restriction digest: | ||
*Needed restriction enzymes are available. | |||
*Thaw buffer | *Thaw buffer | ||
*Waterbath at 37 C is available. (or otherwise, thermomixer, or otherwise, shaking incubator) | |||
| Line 18: | Line 18: | ||
Preparing supercompetent cells: | Preparing supercompetent cells: | ||
*Centrifuge is cooled | *Centrifuge is cooled | ||
*Spectrophotometer and cuvettes for measuring OD are available | |||
*When using spectrophotometer: Correct wavelength is selected (600 nm) | |||
*For snap freezing: Dry ice or liquid nitrogen is available. | *For snap freezing: Dry ice or liquid nitrogen is available. | ||
*Freezer box is available | |||
Revision as of 05:09, 4 May 2013
Checklists for lab procedures:
Transformation:
- Enough plates, with right antibiotic, are available
- Waterbath for heat shock is at right temperature (42 C).
Before heat shock: Place plates in incubator for pre-heating.
Restriction digest:
- Needed restriction enzymes are available.
- Thaw buffer
- Waterbath at 37 C is available. (or otherwise, thermomixer, or otherwise, shaking incubator)
Preparing supercompetent cells:
- Centrifuge is cooled
- Spectrophotometer and cuvettes for measuring OD are available
- When using spectrophotometer: Correct wavelength is selected (600 nm)
- For snap freezing: Dry ice or liquid nitrogen is available.
- Freezer box is available
