User:Jarle Pahr/Checklists

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*After imaging: Save image as image file. Copy file(s) to personal area.
*After imaging: Save image as image file. Copy file(s) to personal area.
*Remove gel from imaging apparatus.
*Remove gel from imaging apparatus.
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'''End of day:'''
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*No samples are left on bench (that shouldn't be there).

Revision as of 13:35, 9 May 2013

Checklists for lab procedures:


Transformation:

  • Supercompetent cells are available (remove from freezer 20 min before use, to avoid waiting for cells to thaw).
  • Enough plates, with right antibiotic, are available. Remove from cold storage, place in incubator for pre-heating.
  • Waterbath for heat shock is at right temperature (42 C).


Before heat shock: Place plates in incubator for pre-heating.


Restriction digest:

  • Needed restriction enzymes are available.
  • Thaw buffer
  • Waterbath at 37 C is available. (or otherwise, thermomixer, or otherwise, shaking incubator)


Ligation:

  • Ligation buffer is available.
  • Ligase is available.
  • Correct ligase is used (T4 DNA ligase)


Preparation of agar plates:

  • Magnetic stirrers are available
  • Antibiotic is available. Thaw antibiotic.


Preparing supercompetent cells:

  • Centrifuge is cooled
  • Spectrophotometer and cuvettes for measuring OD are available
  • When using spectrophotometer: Correct wavelength is selected (600 nm)
  • For snap freezing: Dry ice or liquid nitrogen is available.
  • Freezer box is available

Electrophoresis:

  • GelDoc is operational
  • After imaging: Save image as image file. Copy file(s) to personal area.
  • Remove gel from imaging apparatus.


End of day:

  • No samples are left on bench (that shouldn't be there).
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