User:Jarle Pahr/Buffers
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Qiagen EB buffer: 10 mM Tris-HCl, pH 8.5.
See also http://people.mbi.ucla.edu/sumchan/qiagenbuffer.html
| Buffer | Composition | Use | Storage | Hazardous chemicals? | Comments |
|---|---|---|---|---|---|
| TAE | 40mM Tris, 20mM acetic acid, and 1mM EDTA | Electrophoresis | Room temperature | ||
| Qiagen EB | 10 mM Tris-HCl, pH 8.5 | DNA elution and storage | Room temperature | - | |
| TE (Tris-EDTA) | 10 mM Tris-HCl, pH 8.5 + 10 mM Tris-HCl, pH 8 + 1 mM EDTA | DNA elution and storage | ? | - | EDTA inhbitis nucleases and also inhibits sequencing reactions. |
| NEBuffer 1 | 10mM Bis-Tris-Propane-HCl, 10mM MgCl2,1mM DTT,pH 7.0@25°C | Restriction digestion | -20C | - | CIP shows 50 % activity in NEBuffer 1, compared with 100 % activity in NEBuffers 2-4. |
| NEBuffer 2 | 50mM NaCl,10mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C | Restriction digestion | -20C | - | |
| NEBuffer 3 | 100mM NaCl,50mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C | Restriction digestion | -20C | - | |
| NEBuffer 4 | 50mM Potassium Acetate,20mM Tris-acetate,10mM Magnesium Acetate,1mM DTT,pH 7.9@25°C | Restriction digestion | -20C | - |
Transformation and Storage Solution (TSS):
LB with 10 wt/vol % polyethylene glycol (PEG), 5 % v/v DMSO, 50 mM Mg2+, pH 6.5.
