User:Jarle Pahr/Buffers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jarle Pahr (talk | contribs) No edit summary |
Jarle Pahr (talk | contribs) No edit summary |
||
| Line 10: | Line 10: | ||
| Qiagen EB|| 10 mM Tris-HCl, pH 8.5 || DNA elution and storage || Room temperature || - || | | Qiagen EB|| 10 mM Tris-HCl, pH 8.5 || DNA elution and storage || Room temperature || - || | ||
|- | |- | ||
|TE || 10 mM Tris-HCl, pH 8.5 + 10 mM Tris-HCl, pH 8 + 1 mM EDTA ||DNA elution and storage || ? || - ||EDTA inhbitis nucleases and also inhibits sequencing reactions. | |TE (Tris-EDTA) || 10 mM Tris-HCl, pH 8.5 + 10 mM Tris-HCl, pH 8 + 1 mM EDTA ||DNA elution and storage || ? || - ||EDTA inhbitis nucleases and also inhibits sequencing reactions. | ||
|- | |- | ||
|NEBuffer 1 ||10mM Bis-Tris-Propane-HCl, 10mM MgCl2,1mM DTT,pH 7.0@25°C|| Restriction digestion|| | |NEBuffer 1 ||10mM Bis-Tris-Propane-HCl, 10mM MgCl2,1mM DTT,pH 7.0@25°C|| Restriction digestion||-20C|| - ||CIP shows 50 % activity in NEBuffer 1, compared with 100 % activity in NEBuffers 2-4. | ||
|- | |- | ||
|NEBuffer 2||50mM NaCl,10mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C||Restriction digestion|| | |NEBuffer 2||50mM NaCl,10mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C||Restriction digestion||-20C || - || | ||
|- | |- | ||
|NEBuffer 3||100mM NaCl,50mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C||Restriction digestion|| | |NEBuffer 3||100mM NaCl,50mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C||Restriction digestion||-20C || - || | ||
|- | |- | ||
|NEBuffer 4||50mM Potassium Acetate,20mM Tris-acetate,10mM Magnesium Acetate,1mM DTT,pH 7.9@25°C||Restriction digestion|| | |NEBuffer 4||50mM Potassium Acetate,20mM Tris-acetate,10mM Magnesium Acetate,1mM DTT,pH 7.9@25°C||Restriction digestion||-20C || - || | ||
|} | |} | ||
Revision as of 06:29, 13 March 2013
Qiagen EB buffer: 10 mM Tris-HCl, pH 8.5.
See also http://people.mbi.ucla.edu/sumchan/qiagenbuffer.html
| Buffer | Composition | Use | Storage | Hazardous chemicals? | Comments |
|---|---|---|---|---|---|
| Qiagen EB | 10 mM Tris-HCl, pH 8.5 | DNA elution and storage | Room temperature | - | |
| TE (Tris-EDTA) | 10 mM Tris-HCl, pH 8.5 + 10 mM Tris-HCl, pH 8 + 1 mM EDTA | DNA elution and storage | ? | - | EDTA inhbitis nucleases and also inhibits sequencing reactions. |
| NEBuffer 1 | 10mM Bis-Tris-Propane-HCl, 10mM MgCl2,1mM DTT,pH 7.0@25°C | Restriction digestion | -20C | - | CIP shows 50 % activity in NEBuffer 1, compared with 100 % activity in NEBuffers 2-4. |
| NEBuffer 2 | 50mM NaCl,10mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C | Restriction digestion | -20C | - | |
| NEBuffer 3 | 100mM NaCl,50mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°C | Restriction digestion | -20C | - | |
| NEBuffer 4 | 50mM Potassium Acetate,20mM Tris-acetate,10mM Magnesium Acetate,1mM DTT,pH 7.9@25°C | Restriction digestion | -20C | - |
