User:Jarle Pahr/BLAST: Difference between revisions
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Ian Korf: BLAST: http://www.amazon.com/dp/0596002998/ref=rdr_ext_tmb | Ian Korf: BLAST: http://www.amazon.com/dp/0596002998/ref=rdr_ext_tmb | ||
=Tips= | |||
Selecting a different algorith may be the difference between a hit and a "no significant similarity found" result. If no significant similarity is found at first attempt, try several algorithms. | |||
Revision as of 07:37, 25 April 2013
Notes on NCBI's Basic Local Alignment Search Tool (BLAST) software suite:
Algorithms
Nucleotide blast
blastn:
megablast
Protein blast
BLASTp
Psi-BLAST
Delta-BLAST
Other
blastx: blastx is used to search a protein database using a translated nucleotide sequence query.
tblastn: tblastn is used to search a translated nucleotide database using a protein sequence query.
tblastx: tblastx is used to search a translated nucleotide database using a translated nucleotide sequence query.
Primer-BLAST
Bibliography:
Ian Korf: BLAST: http://www.amazon.com/dp/0596002998/ref=rdr_ext_tmb
Tips
Selecting a different algorith may be the difference between a hit and a "no significant similarity found" result. If no significant similarity is found at first attempt, try several algorithms.
