User:Janet B. Matsen: transformations at room temperature

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Revision as of 18:19, 10 February 2012 by Janet B. Matsen (talk | contribs) (New page: Ever have the urge to transform cells on a Friday, but know you won't be coming in Saturday? Just wait and do it on Monday! I had seen bad contamination when I did this two times. So ...)
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Ever have the urge to transform cells on a Friday, but know you won't be coming in Saturday? Just wait and do it on Monday!

I had seen bad contamination when I did this two times. So I recently tested whether this is an inherent problem in transforming cells at room temperature.

This plate was a control during my last transformation wherein I plated chemically competent Top10 (no plasmids were added) and left on my bench at RT for 3 days. Orange, yellow, & white colonies were observed.

The colonies I transformed at 37oC overnight were perfect, in contrast. It was a fresh set of tips, LB, etc. Also, the batch of Top10 looked great when we plated it overnight at 37oC without antibiotics.

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