User:Jamie Nunziata/Notebook/Protease Research/2015/10/06: Difference between revisions
(fix raw html notebook nav) |
|||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 28: | Line 28: | ||
==Data== | ==Data== | ||
[[Image:JMN_10_6_2015_BlankSample_Fluorescence.jpg]] | |||
[[Image:JMN_10_6_2015_SampleSlope_Fluorescence.jpg]] | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 01:21, 27 September 2017
Project name | Main project page Previous entry Next entry |
ObjectiveThe objective of today's lab was to use a Fluorescence Assay to determine the rate a 1µM solution of a-chymotrypsin degrades AuNP fiber samples. Procedure7 samples of AuNP fibers were spun down at 300rpm for 10 minutes. These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 21.2µL of 47.266µM a-chymotrypsin (in phosphate buffer) and 978.8µL of phosphate buffer was added to each sample tube and a blank Eppindorf tube (no fibers), making a final solution of 1µM a-chymotrypsin.
A full lab procedure can be found in Nicole Bonan's notebook entry for today
Data |