User:Jamie Nunziata/Notebook/Protease Research/2015/09/30

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

The purpose of this lab is to use Bradford Assay to measure a-chymotrypsin degradation at a concentration of 1µM.


Procedure

7 samples of AuNP fibers were spun down at 300rpm for 10 minutes. These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. The fiber samples had 975.6µL of buffer added to them. These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 24.5µL of 40.625µM a-chymotrypsin (in Tris) and 975.4µL of Tris buffer was added to each sample tube an a blank Eppindorf tube (no fibers), making the final solution 1µM a-chymotrypsin.


To each cuvette, the following was added:

  • 1650µL of Tris buffer
  • 750µL of incubated 1µM sample (or blank)
  • 600µL of diluted Bradford Assay in Tris buffer (1:3)


A full lab procedure can be found in Nicole Bonan's notebook entry for today


Absorbance for each cuvette was measure via UV-Vis, and the data is recorded below

Data


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Jamie_Nunziata/Notebook/Protease_Research/2015/09/30&oldid=916631"