User:Jamie Nunziata/Notebook/Protease Research/2015/09/30: Difference between revisions
From OpenWetWare
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Objective== | ==Objective== | ||
The purpose of this lab is to use Bradford Assay to measure | The purpose of this lab is to use Bradford Assay to measure AuNP fiber degradation from a solution of 1µM a-chymotrypsin protease | ||
==Procedure== | ==Procedure== | ||
Revision as of 20:09, 24 November 2015
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveThe purpose of this lab is to use Bradford Assay to measure AuNP fiber degradation from a solution of 1µM a-chymotrypsin protease Procedure7 samples of AuNP fibers were spun down at 300rpm for 10 minutes.These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 24.5µL of 40.625µM a-chymotrypsin (in Tris) and 975.4µL of Tris buffer was added to each sample tube and a blank Eppindorf tube (no fibers), making the final solution 1µM a-chymotrypsin.
Data
| |
