User:Jamie Nunziata/Notebook/Protease Research/2015/09/23

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Objective

The objective for today's lab work is to create variety lysozyme solutions from 1-10µg/mL and to analyze them using a Bradford assay in the UV Vis spectronomer


Procedure

We loosely based our procedure this experiment from the one Dr. Hartings had in his lab notebook.

First was creating a 5050µg/mL lysozyme solution by combining 0.1039g with 5mL of Tris buffer in a volumetric flask. Using the equation M1V1=M2V2, the amount Bradford Assay, lysozyme solution, protein assay reagent, and Tris buffer buffer needed for our various solutions. Those quantities can be found below:



These samples were then run through the Uv Vis spectrometer from 400-800nm for analysis.


Data

The results for the Bradford Assay



Since the calibration curve shows 10µg/mL to be on outlier. Without that value, our R<super>2</super> value greatly increases



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