User:James Chappell: Difference between revisions

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My personal research interest has been the understanding of biological mechanisms within the context of reduced systems, namely in vitro transcription and translation systems. My interest is in learning about other contexts in which reduced systems might find applications. Currently, we are investigating how to utilize such systems to gain insight into RNA function, in particular RNA network motifs.


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I am currently working as a postdoctoral researcher with Prof. Julius Lucks (Lucks Lab) at Cornell University in the School of Chemical and Biomolecular Engineering. I previously obtained a  BSc degree in Biochemistry at Imperial College London, where I continued as a PhD student in Molecular Biosciences under the supervision of Prof. Paul Freemont working at the Imperial College Center for Synthetic Biology and Innovation. My research interests are broadly focused in Synthetic Biology.
 
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===Education and Career===
 
*Post Doc with Prof. Julius Lucks at Chemical and Biomedical Engineering, Cornell University
===Publications===
*PhD with Prof. Paul Freemont at Center for Synthetic Biology and Innovation, Imperial College London <br>
 
*1st Class Honours Bsc in Biochemistry, Imperial College London <br>
1. S. Meyer, J. Chappell, S. Sankar, R Chew, J. B. Lucks* (2015). Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies. Biotechnology and Bioengineering. 36, 154-160.


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2. J. Chappell, K. E. Watters, M. K. Takahashi, J. B. Lucks* (2015). A Renaissance in RNA Synthetic Biology: New Mechanisms, Applications and Tools for the Future. Current Opinion in Chemical Biology. 28, 47-56.
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3. M. K. Takahashi, C. A. Hayes, J. Chappell, Z. Z. Sun, R. M. Murray, V. Noireaux, J. B. Lucks* (2015). Characterizing and Prototyping Genetic Networks with Cell-Free Transcription-Translation Reactions. Methods. Doi: 10.1016/j.ymeth.2015.05.020.
===Scientific Publications===
 
4. J. Chappell, M. K. Takahashi, J. B. Lucks* (2015). Creating Small Transcription Activating RNAs. Nature Chemical Biology, 11, 214-220.
 
5. M. K. Takahashi, J. Chappell, C. A. Hayes, Z. Z. Sun, J. Kim, V. Singhal, K. J. Spring, S. Al-Khabouri, C. P. Fall, V. Noireaux, R. M. Murray, J. B. Lucks* (2014). Rapidly characterizing the fast dynamics of RNA genetic circuitry with in vitro transcription-translation (TX-TL) reactions. ACS Synthetic Biology, 15, 503-515.


<biblio>
6. J. Chappell, M. K. Takahashi, S. Meyer, D. Loughrey, K. E. Watters, J. B. Lucks* (2013). The Centrality of RNA for Engineering Gene Expression. Biotechnology Journal, 8, 1379-1395.
# 1 Chappell,J., Takashashi, M. K.,  Meyer,S., Loughrey, D., Watters, K., Lucks,J. B. ''The Centrality of RNA for Engineering Gene Expression.'' Biotechnology Journal, 2013. DOI: 10.1002/biot.201300018 (FREE)


# 2 Chappell, J., Jensen, K., and Freemont, P.S. (2013). Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Res. [http://www.ncbi.nlm.nih.gov/pubmed/23371936 Pubmed]
7. J. Chappell, K. Jensen, P.S. Freemont* (2013). Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41, 3471-3481.  


# 3 Chappell, J. Freemont, P.S. SYNTHETIC BIOLOGY—A NEW GENERATION OF BIOFILM BIOSENSORS. [http://www.ncbi.nlm.nih.gov/books/NBK84447/ Pubmed Books]
8. J. Chappell, P. S. Freemont* (2013). In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements. Methods in Molecular Biology – Synthetic Biology, 61-74.  


# 4 Gulati1, S. Rouilly, V. Niu1, X. Chappell, J. Kitney, R. Edel, J. Freemont, P. A, deMello.Opportunities for microfluidic technologies in synthetic biology. [http://www.ncbi.nlm.nih.gov/pubmed/19474079 Pubmed]
9. J. Chappell, P. S. Freemont* (2013). Synthetic biology – A new generation of biofilm biosensors


</biblio>
10. S. Gulati, V. Rouilly, X. Niu, J. Chappell, R. I. Kitney, J. B. Edel, P. S. Freemont and A. J. deMello (2009). Opportunities for microfluidic technologies in synthetic biology. Journal of The Royal Society Interface, 6, 493-506
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===Contact Details===
===Contact Details===
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James Chappell, <br>
James Chappell, <br>
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Email - jchappell105@gmail.com
Email - jchappell105@gmail.com
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Revision as of 16:55, 16 November 2015

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James Chappell

Research Interests

I am currently working as a postdoctoral researcher with Prof. Julius Lucks (Lucks Lab) at Cornell University in the School of Chemical and Biomolecular Engineering. I previously obtained a BSc degree in Biochemistry at Imperial College London, where I continued as a PhD student in Molecular Biosciences under the supervision of Prof. Paul Freemont working at the Imperial College Center for Synthetic Biology and Innovation. My research interests are broadly focused in Synthetic Biology.



Publications

1. S. Meyer, J. Chappell, S. Sankar, R Chew, J. B. Lucks* (2015). Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies. Biotechnology and Bioengineering. 36, 154-160.

2. J. Chappell, K. E. Watters, M. K. Takahashi, J. B. Lucks* (2015). A Renaissance in RNA Synthetic Biology: New Mechanisms, Applications and Tools for the Future. Current Opinion in Chemical Biology. 28, 47-56.

3. M. K. Takahashi, C. A. Hayes, J. Chappell, Z. Z. Sun, R. M. Murray, V. Noireaux, J. B. Lucks* (2015). Characterizing and Prototyping Genetic Networks with Cell-Free Transcription-Translation Reactions. Methods. Doi: 10.1016/j.ymeth.2015.05.020.

4. J. Chappell, M. K. Takahashi, J. B. Lucks* (2015). Creating Small Transcription Activating RNAs. Nature Chemical Biology, 11, 214-220.

5. M. K. Takahashi, J. Chappell, C. A. Hayes, Z. Z. Sun, J. Kim, V. Singhal, K. J. Spring, S. Al-Khabouri, C. P. Fall, V. Noireaux, R. M. Murray, J. B. Lucks* (2014). Rapidly characterizing the fast dynamics of RNA genetic circuitry with in vitro transcription-translation (TX-TL) reactions. ACS Synthetic Biology, 15, 503-515.

6. J. Chappell, M. K. Takahashi, S. Meyer, D. Loughrey, K. E. Watters, J. B. Lucks* (2013). The Centrality of RNA for Engineering Gene Expression. Biotechnology Journal, 8, 1379-1395.

7. J. Chappell, K. Jensen, P.S. Freemont* (2013). Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41, 3471-3481.

8. J. Chappell, P. S. Freemont* (2013). In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements. Methods in Molecular Biology – Synthetic Biology, 61-74.

9. J. Chappell, P. S. Freemont* (2013). Synthetic biology – A new generation of biofilm biosensors

10. S. Gulati, V. Rouilly, X. Niu, J. Chappell, R. I. Kitney, J. B. Edel, P. S. Freemont and A. J. deMello (2009). Opportunities for microfluidic technologies in synthetic biology. Journal of The Royal Society Interface, 6, 493-506

Contact Details


James Chappell,
120 Olin Hall,
Chemical and Biomolecular Engineering
Cornell,
NY, USA

Email - jchappell105@gmail.com

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