User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2015/02/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Protein-Templated Quantum Dots</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Protein-Templated Quantum Dots</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Project Summary== | ==Project Summary== | ||
* | * Adapted from [http://onlinelibrary.wiley.com/doi/10.1002/smll.201200760/full Goswami et. al] | ||
* We set out to synthesize mercury-sulfide quantum dots templated by Bovine serum albumin. Multiple attempts proved unsuccessful. However, in the process we discovered a reproducible synthesis that yields, what appears to be, a protein-based film suspended in aqueous solution. This page summarizes all of my experiments conducted during the 2014-2015 academic year, as well as my optimized synthesis procedure for producing these HgS-BSA films (aka the "Quick Synthesis"). | |||
* A few times throughout my notes I refer to the films formed as hydrogels or sol-gels. The sol-gels classification is incorrect, and, from what I can tell working with these materials, hydrogel may be the most appropriate category. | |||
* You will find UV-Vis and Fluorescence data for the first few syntheses (JCS 1-3) in an excel file on DropBox. This file also contains FTIR data from JCS 16. The sample was stuck to the membrane that was used to filter it, so a spectra of the membrane is also included. | |||
* There is a folder containing the DSC data for films JCS 4 and JCS 5 on DropBox. | |||
* You will also find a folder containing powder XRD data for the films produced from JCS 4 and JCS 5. However, something went wrong with the DSC protocol during the runs so these results should be scrutinized. | |||
* While I previously reported preparing a DSC sample with one of the later reactions (I recorded the pan mass, etc.), this sample was never run. The instrument was not working at the time and the sample sat for too long by the time the DSC was up and running. | |||
* A collection of images taken throughout the year have been labeled and uploaded to DropBox as well. | |||
*Note: Un-rotovapped samples that were left sitting in the scintillation vials in the cold room formed a layer of precipitate on the bottom of the glass. The best way to describe these structures is 'shiny aluminum foil.' | |||
==Original Protocol== | ==Original Protocol== | ||
# Prepare at least 5 mL of 15 mg/mL BSA in deionized water. | # Prepare at least 5 mL of 15 mg/mL BSA in deionized water. | ||
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# Slowly add a few mL of DI water while gently swirling the round bottom flask. First, a hydrogel network should form along the sides of the glass as the film rehydrates. | # Slowly add a few mL of DI water while gently swirling the round bottom flask. First, a hydrogel network should form along the sides of the glass as the film rehydrates. | ||
# Slowly add more DI water until the hydrogel detaches from the glass and collapses into sol-gel structures suspended in solution. | # Slowly add more DI water until the hydrogel detaches from the glass and collapses into sol-gel structures suspended in solution. | ||
==Note: These solutions have a tendency to bump on the rotovap. Watch carefully and be ready to break vacuum temporarily in the beginning!== | |||
==Filtration Protocol== | ==Filtration Protocol== | ||
I conducted vacuum filtration once using a membrane filter (Supor-450, 47mm, 0.45μm). This worked to separate the gels from the solution, however '''do not leave the product on the membrane to dry--it will get stuck the membrane.''' | I conducted vacuum filtration once using a membrane filter (Supor-450, 47mm, 0.45μm). This worked to separate the gels from the solution, however '''do not leave the product on the membrane to dry--it will get stuck the membrane.''' |
Latest revision as of 00:55, 27 September 2017
Protein-Templated Quantum Dots | Main project page Previous entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Project Summary
Original Protocol
"Quick" Protocol (15 min Synthesis)
Note: These solutions have a tendency to bump on the rotovap. Watch carefully and be ready to break vacuum temporarily in the beginning!Filtration ProtocolI conducted vacuum filtration once using a membrane filter (Supor-450, 47mm, 0.45μm). This worked to separate the gels from the solution, however do not leave the product on the membrane to dry--it will get stuck the membrane. Summary of Syntheses Attempted
Determining Sol-Gel formation factors
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