User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2015/01/22: Difference between revisions
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== | ==Spring Semester Objectives== | ||
* | This semester will focus on understanding the variables required for protein-gel formation and subsequent characterization of the gels formed during this process. | ||
==Today's Objective== | |||
*Begin a gel synthesis reaction, removing mercury while holding all other conditions constant. | |||
==Protocol (Sample: JCS 13)== | |||
1. 5 mL of 15 mg/mL BSA were prepared (0.0750 g in 5mL of millipore water) | |||
2. An additional 5 mL of DI Water were added to the 5 mL of BSA solution stirring vigorously in a 50 mL round-bottom flask | |||
3. One drop of 1 M NaOH was added to the stirring solution. Test with a pH strip indicated a pH of 10 | |||
4. This began at 2:30 pm. As of right now, the plan is to add 4 ml of 20 mM sodium sulfide tomorrow after stirring for 24 hrs. | |||
==Next Steps== | |||
After adding the sodium sulfide and stirring for an additional 15 minutes, the solution will be dried on the rotovap (utilizing a water bath of approximately 40°C). | |||
The dried residue will be rehydrated to attempt gel-formation. Any resulting structures will be analyzed via DSC and pXRD. | |||
Revision as of 13:27, 22 January 2015
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Spring Semester ObjectivesThis semester will focus on understanding the variables required for protein-gel formation and subsequent characterization of the gels formed during this process. Today's Objective
Protocol (Sample: JCS 13)1. 5 mL of 15 mg/mL BSA were prepared (0.0750 g in 5mL of millipore water) 2. An additional 5 mL of DI Water were added to the 5 mL of BSA solution stirring vigorously in a 50 mL round-bottom flask 3. One drop of 1 M NaOH was added to the stirring solution. Test with a pH strip indicated a pH of 10 4. This began at 2:30 pm. As of right now, the plan is to add 4 ml of 20 mM sodium sulfide tomorrow after stirring for 24 hrs. Next StepsAfter adding the sodium sulfide and stirring for an additional 15 minutes, the solution will be dried on the rotovap (utilizing a water bath of approximately 40°C). The dried residue will be rehydrated to attempt gel-formation. Any resulting structures will be analyzed via DSC and pXRD.
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