User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/24: Difference between revisions
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== | ==Objective== | ||
* | * Complete the QD synthesis begun yesterday | ||
* Continue precipitation of JCS 1 and JCS 2 | |||
* Begin precipitation of JCS 3 | |||
==Protocol== | |||
# Create 20 mM sodium sulfide solution | |||
## .024018 g of Sodium Sulfide (MW=240.18) needed for 5 mL | |||
## Used .1373 g of Sodium Sulfide used (in 5 mL) because the sample is contaminated with excess water | |||
# Add 4 mL of 20 mM sodium sulfide solution to reaction | |||
## Allowed the mixture to stir vigorously for 15 minutes | |||
## Poured contents (minus the stir bar) into a darkened falcon tube and filled with 200 proof ethanol to the 50 mL mark. The falcon tube was left in the freezer to begin precipitation. | |||
## total synthesis time: 13 hrs (including 15 minutes for Sodium Sulfide addition) | |||
# Removed 1 mL aliquot from JCS 3 (before adding ethanol) for future testing with UVVIS and Fluorescence | |||
==Precipitation of JCS 1 and 2== | |||
# Falcon tubes spun at 4000 RPM for 15 minutes | |||
# Solvent was decanted and stored while the dark precipitate was re-suspended in ~15 mL of distilled water. | |||
# Solutions were stored in the dark at room temp for future analysis with UVVIS and fluorescence. | |||
==Notes== | |||
* JCS 1 remained mostly clear with minimal dark precipitate, as it was before it was spun. | |||
* JCS 2 remained darkened brown. After spinning some precipitate was visible | |||
* Eleni and I feel that the next step is to try centrifugation at very high speeds, which was necessary with some of the Mg:ZnS quantum dots we created last semester. | |||
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Revision as of 18:09, 24 September 2014
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Objective
Protocol
Precipitation of JCS 1 and 2
Notes
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