User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/23: Difference between revisions

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(Autocreate 2014/09/23 Entry for User:James_C._Schwabacher/Notebook/Protein-Templated_Quantum_Dots)
 
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==Entry title==
==Objective==
* Insert content here...
* Start new QD synthesis at a lower temperature
* Acquire UVVIS and fluorescence emission data
* Continue precipitation of JCS 1 and JCS 2


==Synthesis Protocol==
# Create 15 mg/mL solution of BSA in HPLC grade water
## .1540 g of BSA in 10.267 mL of water
# Add 5 mL of BSA solution and 5 mL of previously prepared 5mM HgNO<sub>3</sub> solution
# Add 60 μL of ~1M NaOH to reaction while stirring
## pH was 9
# Time reaction started: 12pm


==Precipitation Protocol==
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Revision as of 10:47, 23 September 2014

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Objective

  • Start new QD synthesis at a lower temperature
  • Acquire UVVIS and fluorescence emission data
  • Continue precipitation of JCS 1 and JCS 2

Synthesis Protocol

  1. Create 15 mg/mL solution of BSA in HPLC grade water
    1. .1540 g of BSA in 10.267 mL of water
  2. Add 5 mL of BSA solution and 5 mL of previously prepared 5mM HgNO3 solution
  3. Add 60 μL of ~1M NaOH to reaction while stirring
    1. pH was 9
  4. Time reaction started: 12pm

Precipitation Protocol