User:James C. Schwabacher/Notebook/CHEM-571/2015/04/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==April 1, 2015== | |||
*SDS incorporation | |||
*NOTE: In the end the SDS synthesis did not work. We believe that the surface tension was too low, as the beads readily dissolved as they were dropped into the citric acid/calcium chloride solution. | |||
*Lab cleaning! (Hoods, sinks, benches, etc...) | |||
===Procedure=== | |||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix] and (SDS article)<br.> | |||
<br.> | |||
PVA-Alg-NaMT with SDS | |||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | |||
#* '''1.6 g SDS '''(0.2 wt%), add to water first in '''7 mL of water''' | |||
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' (12.5% PVA and ~0.02% alginate) | |||
#** Add alginate '''while stirring''' PVA-water solution | |||
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable | |||
# Cool dissolved and mixed solution to 35°C | |||
# Prepare 210 mL of citric acid, calcium chloride solution | |||
## Add '''12 g (5%) citric acid''' | |||
## Add '''8.4 g (4%) calcium chloride''' | |||
## Heat solution to 40°C while stirring | |||
# Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip | |||
# Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution | |||
# Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture. | |||
#Allow solution to cool to room temp while stirring for another 20 min | |||
# Stir in '''24 g''' (10%) citric acid solution for 30 min | |||
# Stir in '''14.9 g''' (0.5 M) sodium sulfate solution for 10 min | |||
# Rinse with water to remove excess reactants | |||
# Allow beads to dry in teflon plate overnight | |||
# Clean syringe | |||
#* Pass syringe tip over flame, solution will burst out | |||
#* Use syringe to push water through tip to get any remaining solution out | |||
#* Rinse again with water until solution runs clear | |||
====Measurements==== | |||
*PVA: 1.0 g | |||
*Alg: 0.16 g | |||
*NaMT: 0.11 g | |||
*SDS: 1.62 g | |||
*citric acid: 12.34 g | |||
== | ====Notes==== | ||
*SDS lowered the surface tension too much, and beads did not form | |||
*Friday: absorption, talk to Dr. Fox about XRD data, large scale synthesis | |||
== | |||
* | |||
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Latest revision as of 00:52, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
April 1, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix and (SDS article)<br.> <br.> PVA-Alg-NaMT with SDS
Measurements
Notes
|