User:James C. Schwabacher/Notebook/CHEM-571/2015/03/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2015/03/20 Entry for User:James_C._Schwabacher/Notebook/CHEM-571)
 
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==March 20, 2015==
*repeat synthesis using syringe pump
*prep XRD samples (two types of beads)


===Procedure===
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.>
<br.>
PVA-Alg-NaMT
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate)
#** Add alginate '''while stirring''' PVA-water solution
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of citric acid, calcium chloride solution
## Add '''12 g (5%) citric acid'''
## Add '''8.4 g (4%) calcium chloride'''
## Heat solution to 40°C while stirring
# Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip
# Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution
# Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture.
#Allow solution to cool to room temp while stirring for another 20 min
# Stir in '''24 g''' (10%) citric acid solution for 30 min
# Stir in '''14.9 g''' (0.5 M) sodium sulfate solution for 10 min
# Rinse with water to remove excess reactants
# Allow beads to dry in teflon plate overnight
# Clean syringe
#* Pass syringe tip over flame, solution will burst out
#* Use syringe to push water through tip to get any remaining solution out
#* Rinse again with water until solution runs clear


====Measurements====
*PVA: 1.01 g
*Alg: 0.16 g
*NaMT: 0.1 g
*citric acid: 12.0 g
*CaCl2: 8.44 g
*citric acid: 11.59 + 12.05 g
*sodium sulfate: 14.94 g


 
====Notes====
==Objective==
*Ground up beads for XRD
Learn how to maintain an OpenWetWare Notebook.
 
==Description==
# Add experimental record here. Include what, how, and why...
 
==Data==
* Add data and results here...
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 19:56, 1 April 2015

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

March 20, 2015

  • repeat synthesis using syringe pump
  • prep XRD samples (two types of beads)

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of citric acid, calcium chloride solution
    1. Add 12 g (5%) citric acid
    2. Add 8.4 g (4%) calcium chloride
    3. Heat solution to 40°C while stirring
  4. Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip
  5. Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution
  6. Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture.
  7. Allow solution to cool to room temp while stirring for another 20 min
  8. Stir in 24 g (10%) citric acid solution for 30 min
  9. Stir in 14.9 g (0.5 M) sodium sulfate solution for 10 min
  10. Rinse with water to remove excess reactants
  11. Allow beads to dry in teflon plate overnight
  12. Clean syringe
    • Pass syringe tip over flame, solution will burst out
    • Use syringe to push water through tip to get any remaining solution out
    • Rinse again with water until solution runs clear

Measurements

  • PVA: 1.01 g
  • Alg: 0.16 g
  • NaMT: 0.1 g
  • citric acid: 12.0 g
  • CaCl2: 8.44 g
  • citric acid: 11.59 + 12.05 g
  • sodium sulfate: 14.94 g

Notes

  • Ground up beads for XRD


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:James_C._Schwabacher/Notebook/CHEM-571/2015/03/20&oldid=876777"