User:James C. Schwabacher/Notebook/CHEM-571/2015/03/06: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==March | ==March 6, 2015== | ||
*Repeat synthesis | *Repeat synthesis using a syringe and syringe pump | ||
===Procedure=== | ===Procedure=== | ||
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## Add '''8.4 g (4%) calcium chloride''' | ## Add '''8.4 g (4%) calcium chloride''' | ||
## Heat solution to 40°C while stirring | ## Heat solution to 40°C while stirring | ||
# Add the PVA-Alg-Nanocomposite mixture | # Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip | ||
# Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution | |||
# Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture. | |||
#Allow solution to cool to room temp while stirring for another 20 min | #Allow solution to cool to room temp while stirring for another 20 min | ||
# Stir in '''24 g''' (10%) citric acid solution for 30 min | # Stir in '''24 g''' (10%) citric acid solution for 30 min | ||
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====Measurements==== | ====Measurements==== | ||
*PVA: 1. | *PVA: 1.01 g | ||
*Alg: 0.16 g | *Alg: 0.16 g | ||
*NaMT: 0. | *NaMT: 0.18 g | ||
*citric acid: 12. | *citric acid: 12.98 g | ||
*CaCl2: 8. | *CaCl2: 8.40 g | ||
*citric acid: 24. | *citric acid: 24.50 g | ||
*sodium sulfate: 14.95 g | *sodium sulfate: 14.95 g | ||
==Syringe Pump Settings== | |||
# B-D 10 mL Plastic Syringe | |||
# 20 gauge syringe tip | |||
# Volume: 7.8 | |||
# Rate: 1 mL/min | |||
====Notes==== | ====Notes==== |
Latest revision as of 00:48, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
March 6, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT
Measurements
Syringe Pump Settings
Notes |