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==February 27, 2015== | |||
*Heat citric acid/CaCl<sub>2</sub> solution and use Dr Hartings' smaller syringe tips | |||
===Procedure=== | |||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.> | |||
<br.> | |||
PVA-Alg-NaMT | |||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | |||
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate) | |||
#** Add alginate '''while stirring''' PVA-water solution | |||
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable | |||
# Cool dissolved and mixed solution to 35°C | |||
# Prepare 210 mL of citric acid, calcium chloride solution | |||
## Add '''12-15 g (5-7%) citric acid''' | |||
## Add '''8.4 g (4%) calcium chloride''' | |||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette | |||
## Heat solution to 40°C while stirring for 30 min | |||
## Allow solution to cool to room temp while stirring for another 20 min | |||
#Leave beads in solution, not stirring, overnight | |||
# Stir in '''24 g''' (10%) citric acid solution for 30 min | |||
# Stir in '''14.9 g''' (0.5 M) sodium sulfate solution for 30 min | |||
# Rinse with water to remove excess reactants | |||
# Allow beads to dry in teflon plate overnight | |||
====Measurements==== | |||
*PVA: 1.03 g | |||
*Alg: 0.16 g | |||
*NaMT: 0.1 g | |||
*citric acid: 14.0 g | |||
*CaCl2: 8.44 g | |||
*10% citric acid: 25.0 g | |||
*sodium sulfate: 14.91 g | |||
== | ====Notes==== | ||
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Revision as of 09:27, 6 March 2015
 Biomaterials Design Lab
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February 27, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT
Measurements
Notes | |
