User:James C. Schwabacher/Notebook/CHEM-571/2015/02/20: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==February 20, 2015==
*Get pXRD data > analysis
**Running molding clays
*Rehydrate PVA-Alg-NaMT no citric acid beads in 2 ppm Malachite Green
*Large scale PVA-Alg-NaMT no citric acid synthesis


===Procedure===
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.>
<br.>
PVA-Alg-NaMT (no citric acid)
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate)
##* Add alginate '''while stirring''' PVA-water solution
#* '''~0.1 g of NaMT''' and '''~0.05 g AC''' should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of saturated citric acid with 4% calcium chloride
## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
# Stir for 2 hrs to allow solidification
# Rinse with water to remove excess reactants


 
====Measurements====
==Objective==
Learn how to maintain an OpenWetWare Notebook.
 
==Description==
# Add experimental record here. Include what, how, and why...
 
==Data==
* Add data and results here...
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 




====Notes====
*grinding beads
*fluorescent dye absorption > microscope
*dry beads on slide, keep some wet > microscope
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__NOTOC__
__NOTOC__

Latest revision as of 00:48, 27 September 2017

Biomaterials Design Lab Main project page
Previous entry      Next entry

February 20, 2015

  • Get pXRD data > analysis
    • Running molding clays
  • Rehydrate PVA-Alg-NaMT no citric acid beads in 2 ppm Malachite Green
  • Large scale PVA-Alg-NaMT no citric acid synthesis

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> <br.> PVA-Alg-NaMT (no citric acid)

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT and ~0.05 g AC should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 4% calcium chloride
    1. Add 8.4 g (4%) calcium chloride to 210 mL of water
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Stir for 2 hrs to allow solidification
  6. Rinse with water to remove excess reactants

Measurements

Notes

  • grinding beads
  • fluorescent dye absorption > microscope
  • dry beads on slide, keep some wet > microscope


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