User:James C. Schwabacher/Notebook/CHEM-571/2015/01/28: Difference between revisions

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First trial of synthesizing PVA-Alginate beads with and without NaMT
First trial of synthesizing PVA-Alginate beads with and without NaMT


==Description==
==Protocol==
# Add experimental record here. Include what, how, and why...
*Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C
## 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
## ~0.1 g of nanocomposites should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of saturated citric acid with 2% calcium chloride
## Add 4.2 grams of calcium chloride to 210 mL of water
## Add citric acid to saturation
# Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle
# Stir for 24hrs to allow solidification
# Rinse with water to remove excess reactants


==Data==
==Data==

Revision as of 11:34, 23 January 2015

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Objective

First trial of synthesizing PVA-Alginate beads with and without NaMT

Protocol

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C
    1. 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
    2. ~0.1 g of nanocomposites should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 2% calcium chloride
    1. Add 4.2 grams of calcium chloride to 210 mL of water
    2. Add citric acid to saturation
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle
  5. Stir for 24hrs to allow solidification
  6. Rinse with water to remove excess reactants

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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