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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
First trial of synthesizing PVA-Alginate beads with and without NaMT | |||
== | ===Procedure=== | ||
# Add | Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | ||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | |||
#* 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) | |||
##* Add alginate '''while stirring''' PVA-water solution | |||
#* ~0.1 g of nanocomposites should be added to this mixture where applicable | |||
# Cool dissolved and mixed solution to 35°C | |||
# Prepare 210 mL of saturated citric acid with 2% calcium chloride | |||
## Add 4.2 grams of calcium chloride to 210 mL of water | |||
## Add citric acid to saturation (32 g citric acid) | |||
##* 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid | |||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle | |||
# Stir for 24hrs to allow solidification | |||
# Rinse with water to remove excess reactants | |||
== | ====Measurements==== | ||
* | *PVA-Alg | ||
**1.015 g PVA | |||
**0.1649 g alginate | |||
*PVA-Alg #2 | |||
**0.998 g PVA | |||
**0.159 g Alg | |||
*PVA-Alg-NaMT | |||
**0.999 g PVA | |||
**0.161 g alginate | |||
**0.109 g NaMT | |||
*Saturated citric acid, 2% calcium chloride solution | |||
**4.224 g (PVA-Alg), 4.231 g (PVA-Alg-NaMT), 4.207 g (PVA-Alg #2) calcium chloride | |||
**32.363 g (PVA-Alg), 32.330 g (PVA-Alg-NaMT), 32.267 g (PVA-Alg #2) citric acid | |||
====Notes==== | |||
*PVA-Alg beads pumped with syringe made fiber like clusters | |||
**not cooled at all | |||
**dropped in the middle | |||
*PVA-Alg-NaMT beads made with disposable dropper | |||
**cooled 20 seconds in dropper | |||
**dropped along side | |||
*Turn heat off but keep stirring to prevent clumping pre-bead forming | |||
*Let cool in dropper before dropping | |||
*Move to Dr Fox's automated pumper when repeating bead synthesis | |||
*Use wide dish | |||
*PVA-Alg #2 done in wide dish, dropped with disposable dropper, turn off heat ~1 min before dropping | |||
*Don't fill disposable dropper all the way > cooling solution will come out | |||
==Notes== | ==Notes== | ||
This area is for any observations or conclusions that you would like to note. | This area is for any observations or conclusions that you would like to note. |
Latest revision as of 00:40, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveFirst trial of synthesizing PVA-Alginate beads with and without NaMT ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
Measurements
Notes
NotesThis area is for any observations or conclusions that you would like to note.
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