User:James C. Schwabacher/Notebook/CHEM-571/2014/10/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 26: Line 26:
# Make a calibration curve based on the fluorescence.
# Make a calibration curve based on the fluorescence.
## In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.
## In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.
<u>Finish Films created last week</u>
### Instructions for finishing the films can be seen [[User:Jacob_Esenther/Notebook/581/2014/08/29|here]]


==Data==
==Data==

Revision as of 09:23, 10 October 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

  1. Begin UV-Vis and Fluorescence data collection on Rhodamine6G experimental and standard samples.

Description

Fluorescence

  1. Make stock concentrations (both groups can use the same solutions)
    1. 0.10uM
    2. 0.50uM
    3. 1.0uM
    4. 1.5uM
    5. 2.0uM
  2. Take UV-Vis and Fluorescence spectra of these samples.
  3. Make a calibration curve based on UV-Vis.
    1. Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    1. In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.

Finish Films created last week

      1. Instructions for finishing the films can be seen here

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:James_C._Schwabacher/Notebook/CHEM-571/2014/10/08&oldid=827904"