User:James C. Schwabacher/Notebook/CHEM-571/2014/04/08: Difference between revisions

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==Objective==
# UV-vis and fluorescence of all 6 QD synthesis from 04/02/14.
# Make BSA solution 0.1459mM
# De-gas all solutions
# Synthesis using Gonzalez Method
# Continue precipitation of 78% BSA by mass (second trial)


*BSA made using 0.2409g in 25mL --> 0.1459mM BSA


==Protocol==


==Objective==
This protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/03|Matt Harting's]] notebook, all solutions de-gassed before synthesis.
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==Description==
# To a 3-necked flask is added
# Add experimental record here. Include what, how, and why...
## 25mL of 20 mM L-cysteine (roughly 22% L-Cysteine)
 
## 25mL .1459 mM BSA (roughly 78% by mass)
==Data==
## 5mL of 100mM ZnSO<sub>4</sub> (0.5 mmol)
* Add data and results here...
## 5.437mL of 10.3mM MnCl<sub>2</sub> (0.056 mmol)
## The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
# This mixture is stirred under Ar (I'll use N<sub>2</sub>) for 30 minutes
# 5.505mL of 101mM Na<sub>2</sub>S is quickly added.
# The solution is stirred for 20 minutes
# The solution is then aged at 50C under air for 2 hours.


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
*All solutions were degassed for approximately 10-15 minutes depending on the total volume of the solution
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Miscellaneous]]






[[Category:Course]]
[[Category:Miscellaneous]]




Revision as of 22:56, 20 April 2014

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Objective

  1. UV-vis and fluorescence of all 6 QD synthesis from 04/02/14.
  2. Make BSA solution 0.1459mM
  3. De-gas all solutions
  4. Synthesis using Gonzalez Method
  5. Continue precipitation of 78% BSA by mass (second trial)
  • BSA made using 0.2409g in 25mL --> 0.1459mM BSA

Protocol

This protocol was taken from Matt Harting's notebook, all solutions de-gassed before synthesis.

  1. To a 3-necked flask is added
    1. 25mL of 20 mM L-cysteine (roughly 22% L-Cysteine)
    2. 25mL .1459 mM BSA (roughly 78% by mass)
    3. 5mL of 100mM ZnSO4 (0.5 mmol)
    4. 5.437mL of 10.3mM MnCl2 (0.056 mmol)
    5. The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
  2. This mixture is stirred under Ar (I'll use N2) for 30 minutes
  3. 5.505mL of 101mM Na2S is quickly added.
  4. The solution is stirred for 20 minutes
  5. The solution is then aged at 50C under air for 2 hours.

Notes

  • All solutions were degassed for approximately 10-15 minutes depending on the total volume of the solution






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