User:James C. Schwabacher/Notebook/CHEM-571/2014/02/25: Difference between revisions

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==Description==
==Description==
# Add experimental record here. Include what, how, and why...
The solutions created for tomorrow:
 
# 250mL of 20mM L-cysteine
## FW 121.16g/mol
## measure out: 0.6058g
## measured: 0.6063g
## actual conc: 19.98mM
# 50mL of 100mM ZnSO<sub>4</sub>
## FW 287.56g/mol
## measure out: 1.4378g
## measured: 1.4399 g
## actual conc: 100.1 mM
# 50mL of 10mM MnCl<sub>2</sub>
## FW 197.9g/mol
## measure out: 0.09895g
## measured: 0.0987g
## actual conc: 9.98 mM
# 10mL 1M NaOH
## FW 40.00g/mol
## 0.4284g
## Final concentration a bit over 1M check pH carefully when adjusting!
# 50mL 100mM Na<sub>2</sub>S
## FW 240.18g/mol
## measure out: 1.2009g
## measured: 1.2007g
## actual conc: 100 mM


==Data==
==Data==

Revision as of 13:32, 25 February 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Protocol

  • Run fluorescence spectroscopy for the previously synthesized sets: Cys, GSH, 1-Thioglycerol.
  • Finish precipitation of 80% BSA-CYS sample
  • Start the Sotelo-Gonzalez ("New") method with 80% BSA and Cysteine.

Description

The solutions created for tomorrow:

  1. 250mL of 20mM L-cysteine
    1. FW 121.16g/mol
    2. measure out: 0.6058g
    3. measured: 0.6063g
    4. actual conc: 19.98mM
  2. 50mL of 100mM ZnSO4
    1. FW 287.56g/mol
    2. measure out: 1.4378g
    3. measured: 1.4399 g
    4. actual conc: 100.1 mM
  3. 50mL of 10mM MnCl2
    1. FW 197.9g/mol
    2. measure out: 0.09895g
    3. measured: 0.0987g
    4. actual conc: 9.98 mM
  4. 10mL 1M NaOH
    1. FW 40.00g/mol
    2. 0.4284g
    3. Final concentration a bit over 1M check pH carefully when adjusting!
  5. 50mL 100mM Na2S
    1. FW 240.18g/mol
    2. measure out: 1.2009g
    3. measured: 1.2007g
    4. actual conc: 100 mM

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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