User:James C. Schwabacher/Notebook/CHEM-571/2014/01/29: Difference between revisions
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# Add 200 uL of 40 mM Ammonium Acetate | # Add 200 uL of 40 mM Ammonium Acetate | ||
# Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein | # Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein | ||
# Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL | # Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, <del>and bring volume of water to 2.3 mL</del> | ||
# Incubate at room temp for 1 hour | # Incubate at room temp for 1 hour | ||
# Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing | # Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing | ||
# Incubate for five days at 37 degrees Celsius | # Incubate for five days at 37 degrees Celsius | ||
==Table of BSA:L-Cysteine ratios== | ==Table of BSA:L-Cysteine ratios== |
Revision as of 11:58, 29 January 2014
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||
objectiveThe objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals Preparation of stock solutions
Protocol BSA:L-cysteine Nanocrystals
Table of BSA:L-Cysteine ratios
NotesThis area is for any observations or conclusions that you would like to note.
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