User:Jakob Suckale

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Revision as of 10:00, 21 February 2007

Larger Contributions

50 in total

Clicker in the life sciences (audience response systems) 11/12
Post-publication peer review - 10/12
Word thesis template - 8/12
Biochemistry and cell biology textbooks‎ - 12/11
Glutaraldehyde (Pentanedial) - 11/11

Zotero bibliography software review - 10/11
Protein-protein interaction databases - 9/11
Hydrochloric acid - 6/11
Sex genotyping of mice - 4/11
1 normal acid or base (1N) - 12/10

Gene Construction Kit software review - 12/10
ZEG reporter mouse - 9/10
Goat PTBP1 antibody - 8/10
PCR inhibitors - 7/10
RNase - 7/10

ApE - A Plasmid Editor - 6/10
Nissl staining - 5/10
Gavage error estimation - created 02/10
Bromodeoxyuridine (BrdU) - created 01/10
TE antigen retrieval and immunostaining - created 11/09

Glycerol - created 11/09
Antigen retrieval - extended, reorganised 10/09
Primer Tm estimation methods - 9/09
Critical micelle concentration - 7/09
Significance levels - created 7/09

Making RNA probes with T7 transcription - created 6/09
In vitro transcription with T7 RNA polymerase - restarted 6/09
RNA extraction using self-made guanidinium-acid-phenol reagents - created 2/09
Publication fees - created 1/09
Cell and tissue lysis hub - created 6/08

Generating antibodies - created 4/08
RNA extraction using trizol/tri - 2/08
Finding cDNA clones - created 2/08
Choosing reference genes for qPCR normalisation - created 2/08
QRT-PCR - complete rework, numerous additions 01-05/08

Getting started in Dresden - created 11/07
JC Ledin 2007 Gender gap in science - created 10/07
Papers (bibliography software) - created review of software - 10/07
LaTeX template for PhD thesis - improved template, description, instructions - 9/07
Word vs. LaTeX - *created*, comparison in table, conclusion, links - 8/07 (>2000 hits)

Hardcore PubMeding (advanced PubMed searching) - *created* help page, MeSH, My NCBI,.. - 6/07
How to present your research well [5] - *created*, tips, problems, links - 4/07
guide to Excel for statistics [6] - *created*, common stats functions, t-test, links - 3/07
representing data numerically [7] - *created*, mean/median, sd/sem, examples - 3/07
authorship [8] - *created*, Vancouver & discussion - 3/07
Protein purification tags [9] - *created*, list of tags - 3/07
LacZ staining of cells [10] - *created*, protocol, images - 2/07
Q-PCR - *created*, most now moved to QRT-PCR - 2/07 (>1000 hits)
PCR techniques [11] - overview page *created* - 2/07
PCR [12] - steps of a PCR, primer design, PCR cycle in more readable list form - 12/06

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small contributions

60 in total

Chemical structures drawing software 1/12
Dissecting LSM files 12/11
Significance tests 9/11
2-methylbutane (isopentane) - 3/11
Antibody:LS-C38296 mouse monoclonal PTBP1 - 9/10

Antibody:32-4800 mouse monoclonal Ptbp1 - 9/10
Antibody:PTBP2 AV40965 Sigma - 4/10
Mounting medium - created 4/10
TE - recipe corrected 9/09
Nanodrop - measurement error 7/09

Shipment on dry ice - 7/09
EGTA - created 6/09
SSC - extended 6/09
Antibodies - created 6/09
Bromophenol blue - created 5/09

Xylene cyanol - created 5/09
SDS - created 4/09
Tamoxifen - created 4/09
MOPS - created 3/09
Sterile filters - created 2/09

RNA electrophoresis - additions 1/09
Avoiding RNase contamination - additions 1/09
DEPC - reworked 1/09
Cresol Red - created 1/09
Western blot (immunoblot) hub, Western Blot (immunoblot) - 10/08

Triton - created 10/08
Tween - created 10/08
Antibodypedia - created 10/08
Bioanalyzer - created 8/08
Software for science reference management - created 7/08

Making scientific posters - extended 7/08
RNA blot (Northern) - created 3/08
How to write a research paper - created 3/08
RNA extraction - rework, added 2/08
Scientific publishing - 1/08

Skim - PDF reader and annotation software - 9/07
Started with LaTeX on a Mac - 9/07
LaTeX - rework 9/07
Biosoftware for Mac - 9/07
Powerpoint graphics for science presentations - 5/07

tamoxifen administration to mice - 4/07
EtOH precipitation of nucleic acids 4/07
purification of DNA - 4/07
BMJ Statistics Notes series - 4/07
agarose gel loading dye - 4/07

Hygromycin B - 4/07
mouse tissue lysis for genotyping - 4/07
polymethanal (PFA) - 4/07
JC Lehmann 2006 Measuring scientific quality [13] - 4/07
Journal Club [14] - 4/07

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The Author

Personal background

lecturer biochemistry, University of Tübingen
postdoc in diabetes / neuroscience
PhD in biology/experimental medicine at Max Planck Inst. Cell Biology and Genetics / Med Faculty, Dresden, Deutschland
diploma thesis in bioinformatics - Università degli Studi di Padova, Italia
biochemistry bachelor & master - Oxford University, UK

Drop me a line here on OpenWetWare

Member of the OWW Steering committee

Other wikis

English wikipedia: my user page there

My OWW links

V ppl

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Diagrams & Photos added

8/12	12/11	Glutaraldehyde 11/11	for Zotero review 10/11
Bioanalyzer RNA electropherogram 5/11	male/female genotyping PCR 4/11	3/11	GCK review 12/10
GCK review 12/10	copied from [1] w permission 7/10	7/10 - simple inhibitor illustration based on [2]	6/10 for ApE review
6/10 for ApE review	6/10 for ApE review	5/10 Nissl stain	1/10
Nanodrop 7/09	T7 primer design 6/09	mRNA sense, probe antisense 6/09	made for DNA ex 6/09
modified from PD 6/09	copied from commons 6/09	copied from commons 6/09	copied from commons 6/09
made for agarose protocols 6/09	OWW editing users statistics 1/09	OWW total users statistics 1/09	diagram explaining OWW start site 1/09
SDS; moved from commons 4/09	tamoxifen; moved from commons 4/09	mops dog; copied from commons 3/09	MOPS chemical; copied from commons 3/09
polyclonal antibody generation 4/08	hydrophobicity plot example 4/08	drawing from PDB 4/08	RNA preparation with Trizol - 2/08
to mark open/restricted access - 10/07	to mark open/restricted access - 10/07	uploaded for Papers review - 10/07	uploaded for Skim review article - 9/07
uploaded logo for Latex article - 9/07	created logo for biosoftware for mac page - 9/07	logo for page of the same name about advanced PubMed search functions - 6/07	logo for Powerpoint graphics page - 5/07
Tamoxifen and 4-hydroxytamoxifen structure - 4/07	tube arrangement for DNA precipitation protocol - 4/07	example of small pellet for DNA precipitation protocol - 4/07	made 4 transparent star rating icons for use in OWW, e.g. journal club - 4/07
British Museum, reading room as pickup image for journal club; copied from [3] under CC - 4/07	origami crane as icon for journal club; copied from [4] under CC - 4/07	Tux on slideshow screen - 4/07	sunflower icon moved from wikipedia for use as thanks you icon in discussion - 3/07
logo of R software from R-project.org - 3/07	screenshot of R software running on UNIX from R-project.org - 3/07	coloured B&W graphic from commons for use in stats back link - 3/07	copied from commons for use in ethics back link - 3/07
weak LacZ stain from modified mouse embryonic stem cells, 200x - 2/07	strong LacZ stain from modified mouse embryonic stem cells, 100x - 2/07

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User:Jakob Suckale

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Revision as of 10:00, 21 February 2007

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