User:Jakob Suckale

I'd like to organize a free online course on disease mechanisms. Interested? Vote for it here.

Larger Contributions 59 in total 3D printing in the life sciences 4/16 Google Scholar 3/15 Open peer review 11/14 Sequencing services Germany 9/14 DNA dyes 6/14 Price comparison PCR reagents 6/14 Experimental design and data analysis 3/14 Getting started in Tübingen 10/13 Journals 9/13 Clicker in the life sciences (audience response systems) 11/12 Post-publication peer review - 10/12 Word thesis template - 8/12 Biochemistry and cell biology textbooks‎ - 12/11 Glutaraldehyde (Pentanedial) - 11/11 Zotero bibliography software review - 10/11 Protein-protein interaction databases - 9/11 Hydrochloric acid - 6/11 Sex genotyping of mice - 4/11 1 normal acid or base (1N) - 12/10 Gene Construction Kit software review - 12/10 ZEG reporter mouse - 9/10 Goat PTBP1 antibody - 8/10 PCR inhibitors - 7/10 RNase - 7/10 ApE - A Plasmid Editor - 6/10 Nissl staining - 5/10 Gavage error estimation - created 02/10 Bromodeoxyuridine (BrdU) - created 01/10 TE antigen retrieval and immunostaining - created 11/09 Glycerol - created 11/09 Antigen retrieval - extended, reorganised 10/09 Primer Tm estimation methods - 9/09 Critical micelle concentration - 7/09 Significance levels - created 7/09 Making RNA probes with T7 transcription - created 6/09 In vitro transcription with T7 RNA polymerase - restarted 6/09 RNA extraction using self-made guanidinium-acid-phenol reagents - created 2/09 Publication fees - created 1/09 Cell and tissue lysis hub - created 6/08 Generating antibodies - created 4/08 RNA extraction using trizol/tri - 2/08 Finding cDNA clones - created 2/08 Choosing reference genes for qPCR normalisation - created 2/08 QRT-PCR - complete rework, numerous additions 01-05/08 Getting started in Dresden - created 11/07 JC Ledin 2007 Gender gap in science - created 10/07 Papers (bibliography software) - created review of software - 10/07 LaTeX template for PhD thesis - improved template, description, instructions - 9/07 Word vs. LaTeX - *created*, comparison in table, conclusion, links - 8/07 (>2000 hits) Hardcore PubMeding (advanced PubMed searching) - *created* help page, MeSH, My NCBI,.. - 6/07 How to present your research well [5] - *created*, tips, problems, links - 4/07 guide to Excel for statistics [6] - *created*, common stats functions, t-test, links - 3/07 representing data numerically [7] - *created*, mean/median, sd/sem, examples - 3/07 authorship [8] - *created*, Vancouver & discussion - 3/07 Protein purification tags [9] - *created*, list of tags - 3/07 LacZ staining of cells [10] - *created*, protocol, images - 2/07 Q-PCR - *created*, most now moved to QRT-PCR - 2/07 (>1000 hits) PCR techniques [11] - overview page *created* - 2/07 PCR [12] - steps of a PCR, primer design, PCR cycle in more readable list form - 12/06 edit this panel	small contributions 62 in total 3D printing models of biomolecules 4/16 Price comparison DNA RNA purification kits 6/14 Chemical structures drawing software 1/12 Dissecting LSM files 12/11 Significance tests 9/11 2-methylbutane (isopentane) - 3/11 Antibody:LS-C38296 mouse monoclonal PTBP1 - 9/10 Antibody:32-4800 mouse monoclonal Ptbp1 - 9/10 Antibody:PTBP2 AV40965 Sigma - 4/10 Mounting medium - created 4/10 TE - recipe corrected 9/09 Nanodrop - measurement error 7/09 Shipment on dry ice - 7/09 EGTA - created 6/09 SSC - extended 6/09 Antibodies - created 6/09 Bromophenol blue - created 5/09 Xylene cyanol - created 5/09 SDS - created 4/09 Tamoxifen - created 4/09 MOPS - created 3/09 Sterile filters - created 2/09 RNA electrophoresis - additions 1/09 Avoiding RNase contamination - additions 1/09 DEPC - reworked 1/09 Cresol Red - created 1/09 Western blot (immunoblot) hub, Western Blot (immunoblot) - 10/08 Triton - created 10/08 Tween - created 10/08 Antibodypedia - created 10/08 Bioanalyzer - created 8/08 Software for science reference management - created 7/08 Making scientific posters - extended 7/08 RNA blot (Northern) - created 3/08 How to write a research paper - created 3/08 RNA extraction - rework, added 2/08 Scientific publishing - 1/08 Skim - PDF reader and annotation software - 9/07 Started with LaTeX on a Mac - 9/07 LaTeX - rework 9/07 Biosoftware for Mac - 9/07 Powerpoint graphics for science presentations - 5/07 tamoxifen administration to mice - 4/07 EtOH precipitation of nucleic acids 4/07 purification of DNA - 4/07 BMJ Statistics Notes series - 4/07 agarose gel loading dye - 4/07 Hygromycin B - 4/07 mouse tissue lysis for genotyping - 4/07 polymethanal (PFA) - 4/07 JC Lehmann 2006 Measuring scientific quality [13] - 4/07 Journal Club [14] - 4/07 R Statistics [15] - 3/07 guide to statistics software - 3/07 statistics portal - 3/07 ethics portal - 3/07 quantification of nucleic acids - 2/07 gentamicin - 7/06 aminoglycosides - 7/06 G418 - 7/06 neomycin - 7/06 agarose gel electrophoresis - 6/06 edit this panel	The Author Personal background lecturer in biochemistry, University of Tübingen postdoc in diabetes / neuroscience PhD in biology/experimental medicine at Max Planck Inst. Cell Biology and Genetics / Med Faculty, Dresden, Deutschland masters thesis in bioinformatics - Università degli Studi di Padova, Italia biochemistry undergraduate - Oxford University, UK Drop me a line here on OpenWetWare Member of the OWW Steering committee Other wikis English wikipedia: my user page there My OWW links Reviews OpenWetWare:Ideas novel publishing OpenWetWare:Software OpenWetWare:Information management Special:OWWStats V   ppl edit this panel
Diagrams & Photos added 4/16 4/16 8/12 12/11 Glutaraldehyde 11/11 for Zotero review 10/11 Bioanalyzer RNA electropherogram 5/11 male/female genotyping PCR 4/11 3/11 GCK review 12/10 GCK review 12/10 copied from [1] w permission 7/10 7/10 - simple inhibitor illustration based on [2] 6/10 for ApE review 6/10 for ApE review 6/10 for ApE review 5/10 Nissl stain 1/10 Nanodrop 7/09 T7 primer design 6/09 mRNA sense, probe antisense 6/09 made for DNA ex 6/09 modified from PD 6/09 copied from commons 6/09 copied from commons 6/09 copied from commons 6/09 made for agarose protocols 6/09 OWW editing users statistics 1/09 OWW total users statistics 1/09 diagram explaining OWW start site 1/09 SDS; moved from commons 4/09 tamoxifen; moved from commons 4/09 mops dog; copied from commons 3/09 MOPS chemical; copied from commons 3/09 polyclonal antibody generation 4/08 hydrophobicity plot example 4/08 drawing from PDB 4/08 RNA preparation with Trizol - 2/08 to mark open/restricted access - 10/07 to mark open/restricted access - 10/07 uploaded for Papers review - 10/07 uploaded for Skim review article - 9/07 uploaded logo for Latex article - 9/07 created logo for biosoftware for mac page - 9/07 logo for page of the same name about advanced PubMed search functions - 6/07 logo for Powerpoint graphics page - 5/07 Tamoxifen and 4-hydroxytamoxifen structure - 4/07 tube arrangement for DNA precipitation protocol - 4/07 example of small pellet for DNA precipitation protocol - 4/07 made 4 transparent star rating icons for use in OWW, e.g. journal club - 4/07 British Museum, reading room as pickup image for journal club; copied from [3] under CC - 4/07 origami crane as icon for journal club; copied from [4] under CC - 4/07 Tux on slideshow screen - 4/07 sunflower icon moved from wikipedia for use as thanks you icon in discussion - 3/07 logo of R software from R-project.org - 3/07 screenshot of R software running on UNIX from R-project.org - 3/07 coloured B&W graphic from commons for use in stats back link - 3/07 copied from commons for use in ethics back link - 3/07 weak LacZ stain from modified mouse embryonic stem cells, 200x - 2/07 strong LacZ stain from modified mouse embryonic stem cells, 100x - 2/07 <gallery> edit this panel <html> <center> <a href="http://www3.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/User:Jakob_Suckale" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/User:Jakob_Suckale" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onError="this.onError=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com'" /> </a> </center> </html>