User:Jacob Esenther/Notebook/Chem 571/2015/01/27: Difference between revisions

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==Data==
==Data==
* Add data and results here...
<u>Stock Solutions</u>
* 0.01417g Lysozyme in 50mL H<sub>2</sub>O
* 0.08533g of HAuCl<sub>4</sub> in 100mL H<sub>2</sub>O


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
* Initial reactions will begin as soon as the necessary chemicals arrive to the lab.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]

Revision as of 19:34, 1 February 2015

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Synthesize AuNPs with lysozyme
  • Synthesize AuNP Nanoparticles lysozyme

Description

  1. Stock Solution Prep
    • Lysozyme Stock
      1. 50mL of 20μM Lysozyme Stock solution must be made
      2. 0.01417g of Lysozyme was added to a 50mL volumetric flask and dissolved in H2O
      3. This produced a stock of 19.81μM stock solution
    • Gold Solution Stock
      1. This solution was made by Monika Gasiorek
      2. She added 0.08533g of HAuCl4 to a 100mL volumetric flask and dissolved in water
      3. Our group intends to use 20mL
  2. Preparation for AuNP and AuNP Fibers - Samples are mixed in a test tube, labeled and covered with aluminum foil.
    • AuNP
      1. A 30:1 HAuCl4 to Lysozyme solution must be prepared
      2. 2.095mL of Lysozyme solution, 0.5mL of HAuCl4 solution, and 2.405mL H2O
    • AuNP Fibers
      1. A 45:1 HAuCl4 to Lysozyme solution must be prepared
      2. 1.40mL of Lysozyme solution, 0.5mL of HAuCl4 solution, and 3.1mL H2O
  3. Synthesis of AuNP and AuNP Fibers
    • Test tubes were placed into an oven and cooked for 4 hours
    • Samples to be retrieved tomorrow

Data

Stock Solutions

  • 0.01417g Lysozyme in 50mL H2O
  • 0.08533g of HAuCl4 in 100mL H2O

Notes

  • Initial reactions will begin as soon as the necessary chemicals arrive to the lab.




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