User:Jacob Esenther/Notebook/Chem 571/2015/01/27

Biomaterials Design Lab

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Objective Synthesize AuNPs with lysozyme Synthesize AuNP Nanoparticles lysozyme Description Stock Solution Prep Lysozyme Stock 50mL of 20μM Lysozyme Stock solution must be made 0.01417g of Lysozyme was added to a 50mL volumetric flask and dissolved in H2O This produced a stock of 19.81μM stock solution Gold Solution Stock This solution was made by Monika Gasiorek She added 0.08533g of HAuCl4 to a 100mL volumetric flask and dissolved in water Our group intends to use 20mL Preparation for AuNP and AuNP Fibers - Samples are mixed in a test tube, labeled and covered with aluminum foil. AuNP A 30:1 HAuCl4 to Lysozyme solution must be prepared 2.095mL of Lysozyme solution, 0.5mL of HAuCl4 solution, and 2.405mL H2O AuNP Fibers A 45:1 HAuCl4 to Lysozyme solution must be prepared 1.40mL of Lysozyme solution, 0.5mL of HAuCl4 solution, and 3.1mL H2O Synthesis of AuNP and AuNP Fibers Test tubes were placed into an oven and cooked for 4 hours Samples to be retrieved tomorrow Data Stock Solutions 0.01417g Lysozyme in 50mL H2O 0.08533g of HAuCl4 in 100mL H2O Notes Initial reactions will begin as soon as the necessary chemicals arrive to the lab.