User:Jacob Esenther/Notebook/Chem 571/2014/10/22: Difference between revisions
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==Objective== | ==Objective== | ||
# Ran Titration Analysis on KI-Colloid dialysis | |||
# Ran Bradford Analysis on KI-Colloid dialysis | |||
==Description== | ==Description== | ||
# | # Titration | ||
## Added 3mL 1M HNO<sub>3</sub> and 0.5mL KI solution to 10mL DI H<sub>2</sub>O | |||
## Added 2 mL AgNO<sub>3</sub> and swirl to complete precipitation | |||
## Added 200 μL Ferric alum indicator | |||
## Titrate with your NH<sub>4</sub>SCN solution | |||
# Ran Bradford Analysis on KI-Colloid dialysis | |||
## As expected, the Bradford analysis held a constant protein concentration | |||
##* Lysozyme is too big to pass through the 3500 MWCO so it was constant on one side | |||
##* KI is not expected to react with lysozyme so protein concentration was not affected | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 12:41, 22 October 2014
 Biomaterials Design Lab
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Objective
Description
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