User:Jacob Esenther/Notebook/Chem 571/2014/10/14

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Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  1. Extracted contents of the HCl Lysozyme dialysis
    1. These were placed into 1.5mL centrifuge tubes for further testing
  2. Ran titrations
    1. Michael preformed the titrations
    2. The procedure may be viewed on Dr.Fox's page here.
  3. Created new SDS Page Solutions
  4. Created a Potassium Phosphate Buffer
    1. The original protocol asked to make the buffer using KH2PO4 and 1M KOH
    2. We opted to use the monobasic and dibasic species of the molecule to obtain the desired pH of 6.24

Data

To make the Potassium Phosphate buffer we used:

  • 0.0839g of K2HPO4
  • 0.2888g of KHPO4

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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