User:Jacob Esenther/Notebook/Chem 571/2014/10/14: Difference between revisions
From OpenWetWare
| Line 15: | Line 15: | ||
==Description== | ==Description== | ||
# Extracted contents of the HCl Lysozyme dialysis | # Extracted contents of the HCl Lysozyme dialysis | ||
## These were placed into 1.5mL centrifuge tubes for further testing | |||
# Ran titrations | # Ran titrations | ||
## Michael preformed the titrations | |||
## The procedure may be viewed on Dr.Fox's page [[User:Douglas_M._Fox/Notebook/AU_CHEM-571_F2011_Lab_Support/2014/10/08|here]]. | |||
# Created new SDS Page Solutions | # Created new SDS Page Solutions | ||
# Created a Potassium Phosphate Buffer | # Created a Potassium Phosphate Buffer | ||
## The original protocol asked to make the buffer using KH<sub>2</sub>PO<sub>4</sub> and 1M KOH | |||
## We opted to use the monobasic and dibasic species of the molecule to obtain the desired pH of 6.24 | |||
##* Calculations for the buffer were made using this website [[http://www.liv.ac.uk/buffers/buffercalc.html|The University of Liverpool Buffer Calculator]] | |||
==Data== | ==Data== | ||
Revision as of 11:36, 14 October 2014
 Biomaterials Design Lab
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
ObjectiveLearn how to maintain an OpenWetWare Notebook. Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
| |
