User:Jacob Esenther/Notebook/Chem 571/2014/09/23: Difference between revisions
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## Loaded 20uL of protein ladder into column 1 of the gel | ## Loaded 20uL of protein ladder into column 1 of the gel | ||
## Loaded 20uL of samples into the appropriate lane of the gel | ## Loaded 20uL of samples into the appropriate lane of the gel | ||
# Performed electrophoresis | # Performed electrophoresis | ||
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==Data== | ==Data== | ||
* | * Well Number | ||
** 1 - Protein Ladder | |||
** 4 - BSA Stock Solution | |||
** 6 - Soy Stock Solution | |||
** 8 - Lysozyme Colloid | |||
** 10 - Lysozyme Stock Solution | |||
** 12 - BSA Colloid | |||
==Notes== | ==Notes== |
Revision as of 10:48, 23 September 2014
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Objective
DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data
NotesThis area is for any observations or conclusions that you would like to note.
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