User:Jacob Esenther/Notebook/Chem 571/2014/09/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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# Bradford Assay of the Lysozyme Stock | # Bradford Assay of the Lysozyme Stock | ||
## 0.50350g of Lysozyme was added to 1L of 0.90378% saline solution | ## 0.50350g of Lysozyme was added to 1L of 0.90378% saline solution | ||
## Tris-NaCl buffer prepared with 1.5145g Tris,0.7291g NaCl, and 250mL H<sub>2</sub>O | |||
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** 12 - BSA Colloid | ** 12 - BSA Colloid | ||
*Bradford | |||
{| | |||
| align="center" style="background:#f0f0f0;"|'''Dilution #''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''Initial Lysosyme (μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Bradford (μL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Bradford (μL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final Lysozyme (μg/mL)''' | |||
|- | |||
| 1||||503.500||800||200||402.800 | |||
|- | |||
| 2||||251.750||800||200||201.400 | |||
|- | |||
| 3||||125.875||800||200||100.700 | |||
|- | |||
| 4||||62.938||800||200||50.350 | |||
|- | |||
| 5||||31.469||800||200||25.175 | |||
|- | |||
| 6||||15.734||800||200||12.588 | |||
|- | |||
| 7||||7.867||800||200||6.294 | |||
|- | |||
| 8||||3.934||800||200||3.147 | |||
|- | |||
| 9||||1.967||800||200||1.573 | |||
|- | |||
| | |||
|} | |||
==Notes== | |||
* Bradford Assay is only effective from 1μg/mL to 10μg/mL | |||
* The tape was not removed from the electrophoresis, so the gel was not successful. This data will be borrowed from Monika and Paul. | |||
[[Category:Course]] | [[Category:Course]] |
Latest revision as of 00:19, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Objective
DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data
Notes
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