User:Jacob Esenther/Notebook/Chem 571/2014/09/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
# Dialysis of protein and colloid against different dialyzing solutions | |||
==Description== | ==Description== | ||
# | # Dialysis | ||
## prepared 500mL of 50mM NaCl | |||
## prepared 1L of 50mM Glycine buffer, pH 3.5 | |||
##* used 0.4mL 0.95M HCl and 0.37513 g glycine | |||
## Prepared 25mL of 1g/L Lysozyme | |||
## Dialyzed 4mL of lysozyme in about 3 inches of dialysis tubing | |||
##* prepared a 3500 g/mol MWCO tube for NaCl, Glycine, and HPLC water soaks | |||
##* prepared a 25k g/mol MWCO tube for Glycine Soak | |||
## also dialyzed 4mL of a colloid solution in 3500 g/mol MWCO tube for Glycine soak | |||
# Creating Calibration Curves | |||
## Calibration curves of pH and conductivity were created for both NaCl and Glycine buffer | |||
##* 15mL of 2x, 5x, 10x, 50x, 100x, 200x, and 500x dilutions | |||
==Data== | ==Data== |
Latest revision as of 00:27, 27 September 2017
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Objective
Description
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