User:Jackl/Network mutants: Difference between revisions
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus= | | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=nrg1 Nrg1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=pdr1 Pdr1]|| || || || || || || || || || | ||
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Revision as of 21:58, 15 September 2006
What is this?
This is mostly just a way for me to keep track of work I'm doing to make network mutants...last updated 08 Aug 2006.
Network Mutants: Set 1
| Name | Input / Output | PCR | Transform (wt) | Colony PCR check | Transform Cre | Recombine | Colony PCR check | Cure plasmid | Final check | Mutator | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|
| j01 | Rap1 / Sip4 | ||||||||||
| j02 | Rpn4 / Pdr1 | ||||||||||
| j03 | Skn7 / Aft2 | ||||||||||
| j04 | Leu3 / Met4 | ||||||||||
| j05 | Cbf1 / Reb1 | ||||||||||
| j06 | Sum1 / Gcr1 | ||||||||||
| j07 | Yap1 / Aft2 | ||||||||||
| j08 | Phd1 / Cin5 | ||||||||||
| j09 | Hap4 / Put3 | ||||||||||
| j10 | Abf1 / Put3 | ||||||||||
| j11 | Reb1 / Gts1 | ||||||||||
| j12 | Ino2 / Opi1 | ||||||||||
| j13 | Abf1 / Pho2 | ||||||||||
| j14 | Abf1 / Dal81 | ||||||||||
| j15 | Ash1 / Yhp1 | ||||||||||
| j16 | Rpn4 / Reb1 | ||||||||||
| j17 | Nrg1 / Pdr1 | ||||||||||
| j18 | Cbf1 / Swi4 | ||||||||||
| j19 | Fkh1 / Ste12 | ||||||||||
| j20 | Mcm1 / Swi4 | ||||||||||
| j21 | Yap6 / Dal81 |
Network Mutants: Set 2
| Name | Input / Output | PCR | Transform (wt) | Colony PCR check | Transform Cre | Recombine | Colony PCR check | Cure plasmid | Final check | Mutator | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|
| t01 | Cin5 / Rox1 | ||||||||||
| t02 | Cin5 / Yap6 | ||||||||||
| t03 | Gcn4 / Put3 | ||||||||||
| t04 | Mac1 / Cup9 | ||||||||||
| t05 | Phd1 / Msn4 | ||||||||||
| t06 | Gcn4 / Leu3 | ||||||||||
| t07 | Gcn4 / Met4 | ||||||||||
| t08 | Ste12 / Dig1 | ||||||||||
| t09 | Ndd1 / Swi5 | ||||||||||
| t10 | Skn7 / Nrg1 | ||||||||||
| t11 | Dal82 / Uga3 | ||||||||||
| t12 | Cbf1 / Rpn4 | ||||||||||
| t13 | Hsf1 / Rpn4 | ||||||||||
| t14 | Cha4 / Sum1 | ||||||||||
| t15 | Ste12 / Sum1 | ||||||||||
| t16 | Hap1 / Rgt1 | ||||||||||
| t17 | Rox1 / Rox1 | ||||||||||
| t18 | Arg81 / Arg81 | ||||||||||
| t19 | Sum1 / Sum1 | ||||||||||
| t20 | Yap7 / Yap7 | ||||||||||
| t21 | Nrg1 / Nrg1 | ||||||||||
| t22 | Cin5 / Cin5 |
Explanation...
What does everything mean??
- Name: what I call the network mutant
- Input / Output: the input and output nodes for the link I'm removing
- Notes: stuff I should remember
The following headings track my progress throug the creation of network mutants. Green boxes signify completed work and yellow boxes signify work in progress
- PCR: if I've succcessfully made the PCR product for homologous recombination
- Transform (wt): if I've successfully transformed the PCR product into wildtype yeast
- Colony PCR Check: if I've confirmed the transformation via colony PCR
- Transform Cre: if I've transformed the Cre plasmid (into yeast with the aforementioned PCR product)
- Recombine: if I've induced Cre expression and recombined out the selective marker on the PCR product
- Colony PCR Check: if I've confirmed what I have up to this point via colony PCR
- Cure plasmid: if I've cured the yeast mutant of the Cre plasmid
- Final check: if I've checked the final network mutant (via growth on plates and/or sequencing
- Mutator: if I've made a mutator of the network mutant (by deleting msh2)
