User:Jackl/Network mutants: Difference between revisions

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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=leu3 Leu3] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=met4 Met4]|| bgcolor="blue"| || bgcolor="blue"| || || bgcolor="blue"| || bgcolor="blue"| || || bgcolor="blue"| || || bgcolor="blue"| ||  
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=leu3 Leu3] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=met4 Met4]|| bgcolor=#00FF00| || bgcolor=#00FF00| || || bgcolor=#00FF00| || bgcolor=#00FF00| || || bgcolor=#00FF00| || || bgcolor=#00FF00| ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=abf1 Abf1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=put3 Put3]|| bgcolor=#00FF00| || bgcolor="yellow"| || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ino2 Ino2] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=opi1 Opi1]|| bgcolor="blue"| || bgcolor="yellow"| || || || || || || || ||  
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ino2 Ino2] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=opi1 Opi1]|| bgcolor=#00FF00| || bgcolor="yellow"| || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=abf1 Abf1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=dal81 Dal81]|| || || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ash1 Ash1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=yhp1 Yhp1]|| || || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=rpn4 Rpn4] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=reb1 Reb1]|| || || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=arg1 Arg1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=pdr1 Pdr1]|| || || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=cbf1 Cbf1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi4 Swi4]|| || || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=fkh1 Fkh1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ste12 Ste12]|| || || || || || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mcm1 Mcm1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi4 Swi4]|| bgcolor="blue"| || bgcolor="blue"| || || bgcolor="blue"| ||  bgcolor="yellow"| || || || || ||  
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mcm1 Mcm1] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi4 Swi4]|| bgcolor=#00FF00| || bgcolor=#00FF00| || || bgcolor=#00FF00| ||  bgcolor="yellow"| || || || || ||  
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| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=yap6 Yap6] / [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=dal81 Dal81]|| || || || || || || || || ||  
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Revision as of 17:04, 3 August 2006

What is this?

This is mostly just a way for me to keep track of work I'm doing to make network mutants...last updated 03 Aug 2006.

Network Mutants: Set 1

Name Input / Output PCR Transform (wt) Colony PCR check Transform Cre Recombine Colony PCR check Cure plasmid Final check Mutator Notes
j01 Rap1 / Sip4
j02 Rpn4 / Pdr1
j03 Skn7 / Aft2
j04 Leu3 / Met4
j05 Cbf1 / Reb1
j06 Sum1 / Gcr1
j07 Yap1 / Aft2
j08 Phd1 / Cin5
j09 Hap4 / Put3
j10 Abf1 / Put3
j11 Reb1 / Gts1
j12 Ino2 / Opi1
j13 Abf1 / Pho2
j14 Abf1 / Dal81
j15 Ash1 / Yhp1
j16 Rpn4 / Reb1
j17 Arg1 / Pdr1
j18 Cbf1 / Swi4
j19 Fkh1 / Ste12
j20 Mcm1 / Swi4
j21 Yap6 / Dal81

Network Mutants: Set 2

Name Input / Output PCR Transform (wt) Colony PCR check Transform Cre Recombine Colony PCR check Cure plasmid Final check Mutator Notes
t01 Cin5 / Rox1
t02 Cin5 / Yap6
t03 Gcn4 / Put3
t04 Mac1 / Cup9
t05 Phd1 / Msn4
t06 Gcn4 / Leu3
t07 Gcn4 / Met4
t08 Ste12 / Dig1
t09 Ndd1 / Swi5
t10 Skn7 / Nrg1
t11 Dal82 / Uga3
t12 Cbf1 / Rpn4
t13 Hsf1 / Rpn4
t14 Cha4 / Sum1
t15 Ste12 / Sum1
t16 Hap1 / Rgt1
t17 Rox1 / Rox1
t18 Arg81 / Arg81
t19 Sum1 / Sum1
t20 Yap7 / Yap7
t21 Nrg1 / Nrg1
t22 Cin5 / Cin5

Explanation...

What does everything mean??

  • Name: what I call the network mutant
  • Input / Output: the input and output nodes for the link I'm removing
  • Notes: stuff I should remember

The following headings track my progress throug the creation of network mutants. Blue boxes signify completed work and yellow boxes signify work in progress

  • PCR: if I've succcessfully made the PCR product for homologous recombination
  • Transform (wt): if I've successfully transformed the PCR product into wildtype yeast
  • Colony PCR Check: if I've confirmed the transformation via colony PCR
  • Transform Cre: if I've transformed the Cre plasmid (into yeast with the aforementioned PCR product)
  • Recombine: if I've induced Cre expression and recombined out the selective marker on the PCR product
  • Colony PCR Check: if I've confirmed what I have up to this point via colony PCR
  • Cure plasmid: if I've cured the yeast mutant of the Cre plasmid
  • Final check: if I've checked the final network mutant (via growth on plates and/or sequencing
  • Mutator: if I've made a mutator of the network mutant (by deleting msh2)
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