User:J. Steen Hoyer: Difference between revisions
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*Computational and Systems Biology Program, Washington University in St. Louis | *Computational and Systems Biology Program, Washington University in St. Louis | ||
*[[Special:Emailuser/J. Steen Hoyer|Email me through OpenWetWare]] | *[[Special:Emailuser/J. Steen Hoyer|Email me through OpenWetWare]] | ||
*http://orcid.org/0000-0002-1338-8900 | |||
I previously worked in the Kaplinsky lab at Swarthmore College and the Meyers lab at the University of Delaware. | I previously worked in the Kaplinsky lab at Swarthmore College and the Meyers lab at the University of Delaware. I was in the Molecular and Cellular Biology Program at Oregon State University prior to transferring to WUSTL. | ||
==Links and notes== | ==Links and notes== | ||
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===S. cerevisiae=== | ===S. cerevisiae=== | ||
Fred Sherman: | Clontech Yeast Protocols Handbook [http://www.clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17602&minisite=10020&secItmId=16116 PDF], Matchmaker Handbook [http://www.clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17604&minisite=10020&secItmId=16120 PDF] | ||
Fred Sherman (RIP): | |||
[http://www.urmc.rochester.edu/biochemistry-biophysics/images/Saccharomyces-Cerevisiae-Yeast-Intro.pdf Intro], | |||
[http://www.urmc.rochester.edu/biochemistry-biophysics/images/Getting-Started-With-Yeast.pdf how-to-start] | |||
Different names for dropout media | Different names for dropout media | ||
* SC = synthetic complete. | * [[SC]] = synthetic complete. | ||
* SD = synthetic dropout/dextrose | * [[SD]] = synthetic dropout/dextrose | ||
Some people (e.g. Sherman) reserve the term 'SD' for minimal media without amino acids, and refer to the corresponding media with amino acids as 'SC'. Others (e.g. Clontech) refer to both as 'SD'. Still others refer to these (I think) as 'CSM' or 'CM'. | |||
Abbreviation 1 for Yeast Extract/Peptone/Dextrose (YEPD) is easy to confuse with YEP media, and the abbreviation 2 (YPDA [with Adenine]) is easy to confuse with Potato Dextrose Agar (PDA) media! | |||
Some people use 'YCM', which seems to just be YPD with 1% peptone instead of 2% peptone. Why? | |||
Doubling times in YPD typically about 90 minutes, reaching stationary phase at around 16-18 hours. | |||
Doubling times in synthetic media typically about 140 minutes, reaching stationary phase at around 32-36 hours. | |||
[[McClean:Protocols]] | [[McClean:Protocols]] | ||
Revision as of 13:10, 11 March 2014
Contact Info
- J. Steen Hoyer
- Carrington Lab, Donald Danforth Plant Science Center
- Computational and Systems Biology Program, Washington University in St. Louis
- Email me through OpenWetWare
- http://orcid.org/0000-0002-1338-8900
I previously worked in the Kaplinsky lab at Swarthmore College and the Meyers lab at the University of Delaware. I was in the Molecular and Cellular Biology Program at Oregon State University prior to transferring to WUSTL.
Links and notes
S. cerevisiae
Clontech Yeast Protocols Handbook PDF, Matchmaker Handbook PDF
Fred Sherman (RIP): Intro, how-to-start
Different names for dropout media
Some people (e.g. Sherman) reserve the term 'SD' for minimal media without amino acids, and refer to the corresponding media with amino acids as 'SC'. Others (e.g. Clontech) refer to both as 'SD'. Still others refer to these (I think) as 'CSM' or 'CM'.
Abbreviation 1 for Yeast Extract/Peptone/Dextrose (YEPD) is easy to confuse with YEP media, and the abbreviation 2 (YPDA [with Adenine]) is easy to confuse with Potato Dextrose Agar (PDA) media!
Some people use 'YCM', which seems to just be YPD with 1% peptone instead of 2% peptone. Why?
Doubling times in YPD typically about 90 minutes, reaching stationary phase at around 16-18 hours. Doubling times in synthetic media typically about 140 minutes, reaching stationary phase at around 32-36 hours.
